Browsing by Subject "blood"
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Item Antibody-Independent Isolation of Circulating Tumor Cells by Continuous-Flow Dielectrophoresis(2013-01) Shim, Sangjo; Stemke-Hale, Katherine; Tsimberidou, Apostolia M.; Noshari, Jamileh; Anderson, Thomas E.; Gascoyne, Peter R. C.; Shim, Sangjo; Noshari, Jamileh; Anderson, Thomas E.; Gascoyne, Peter R. C.Circulating tumor cells (CTCs) are prognostic markers for the recurrence of cancer and may carry molecular information relevant to cancer diagnosis. Dielectrophoresis (DEP) has been proposed as a molecular marker-independent approach for isolating CTCs from blood and has been shown to be broadly applicable to different types of cancers. However, existing batch-mode microfluidic DEP methods have been unable to process 10 ml clinical blood specimens rapidly enough. To achieve the required processing rates of 106 nucleated cells/min, we describe a continuous flow microfluidic processing chamber into which the peripheral blood mononuclear cell fraction of a clinical specimen is slowly injected, deionized by diffusion, and then subjected to a balance of DEP, sedimentation and hydrodynamic lift forces. These forces cause tumor cells to be transported close to the floor of the chamber, while blood cells are carried about three cell diameters above them. The tumor cells are isolated by skimming them from the bottom of the chamber while the blood cells flow to waste. The principles, design, and modeling of the continuous-flow system are presented. To illustrate operation of the technology, we demonstrate the isolation of circulating colon tumor cells from clinical specimens and verify the tumor origin of these cells by molecular analysis. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4774304]Item CT CBV Perfusion Mapping As A Sign of Futility In Acute Ischemic Stroke(2016-04) Rizvi, Imran; Ahmed, Yousuf; Rutledge, NealItem El pueblo que no da respiro(2007-11-08) Pagina 12Item Failure of Sterne- and Pasteur-Like Strains of Bacillus anthracis to Replicate and Survive in the Urban Bluebottle Blow Fly Calliphora vicina under Laboratory Conditions(PLOS One, 2014-01-02) von Terzi, Britta; Turnbull, Peter C.; Bellan, Steve E.; Beyer, Wolfgang -Institute of Environmental and Animal Hygiene, Stuttgart, GermanyThis study aimed to elucidate the bacteriological events occurring within the gut of Calliphora vicina, selected as the European representative of blow flies held responsible for the spread of anthrax during epidemics in certain parts of the world. Green-fluorescent-protein-carrying derivatives of Bacillus anthracis were used. These lacked either one of the virulence plasmids pXO1 and pXO2 and were infected, or not infected, with a worm intestine phage (Wip4) known to influence the phenotype and survival of the pathogen. Blood meals were prepared for the flies by inoculation of sheep blood with germinated and, in case of pXO2+ strains, encapsulated cells of the four B. anthracis strains. After being fed for 4 h an initial 10 flies were externally disinfected with peracetic acid to ensure subsequent quantitation representing ingested B. anthracis only. Following neutralization, they were crushed in sterile saline. Over each of the ensuing 7 to 10 days, 10 flies were removed and processed the same way. In the absence of Wip4, strains showed steady declines to undetectable in the total B. anthracis counts, within 7–9 days. With the phage infected strains, the falls in viable counts were significantly more rapid than in their uninfected counterparts. Spores were detectable in flies for longer periods than vegetative bacteria. In line with the findings in both biting and non-biting flies of early workers our results indicate that B. anthracis does not multiply in the guts of blow flies and survival is limited to a matter of days.Item Interesado, Defensor del Pueblo, Dictamen, March 29, 2007(2007-03-29) Vitti, Jeronimo; Maiztegui, CristinaItem Para Conocimiento de la Jefa del Area III, May 30, 2008(2008-05-30) De Francesco, VirginiaItem Plan de Remediacion Ambiental Abra Pampa-Subprograma Gestion Ambiental Minera(0000-00-00) Gobierno de Jujuy