Specific Adhesion of Membranes Simultaneously Supports Dual Heterogeneities in Lipids and Proteins

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dc.creatorShindell, O.
dc.creatorMica, N.
dc.creatorRitzer, M.
dc.creatorGordon, Vernita D.
dc.date.accessioned2016-07-14T17:56:22Z
dc.date.available2016-07-14T17:56:22Z
dc.date.issued2015-03en
dc.descriptionThe University of Texas at Austin, Department of Physics and Center for Nonlinear Dynamics, 2515 Speedway, Stop C1610, Austin, Texas 78712-1199, USA.en_US
dc.description.abstractMembrane adhesion is a vital component of many biological processes. Heterogeneities in lipid and protein composition are often associated with the adhesion site. These heterogeneities are thought to play functional roles in facilitating signalling. Here we experimentally examine this phenomenon using model membranes made of a mixture of lipids that is near a phase boundary at room temperature. Non-adherent model membranes are in a well-mixed, disordered-fluid lipid phase indicated by homogeneous distribution of a fluorescent dye that is a marker for the fluid-disordered (Ld) phase. We specifically adhere membranes to a flat substrate bilayer using biotin–avidin binding. Adhesion produces two types of coexisting heterogeneities: an ordered lipid phase that excludes binding proteins and the fluorescent membrane dye, and a disordered lipid phase that is enriched in both binding proteins and membrane dye compared with the non-adhered portion of the same membrane. Thus, a single type of adhesion interaction (biotin–avidin binding), in an initially-homogeneous system, simultaneously stabilizes both ordered-phase and disordered-phase heterogeneities that are compositionally distinct from the non-adhered portion of the vesicle. These heterogeneities are long-lived and unchanged upon increased temperature.en_US
dc.description.departmentCenter for Nonlinear Dynamicsen_US
dc.description.sponsorshipThis work was funded by start-up funds from The University of Texas at Austin (UT Austin) to VDG. MR was supported in part by undergraduate research fellowships from UT Austin. We are grateful to Professor Jeanne Stachowiak (Biomedical Engineering, UT Austin) for helpful conversations about membrane formation and to her and her group for technical assistance. We thank Professor Ernst-Ludwig Florin (Physics, UT Austin) for the extruder and for cover glasses. We thank Professor Lauren Ehrlich (Molecular Biosciences, UT Austin) for helpful conversations about the immune synapse.en_US
dc.identifierdoi:10.15781/T2V40K007
dc.identifier.citationShindell, O., N. Mica, M. Ritzer, and V. D. Gordon. "Specific adhesion of membranes simultaneously supports dual heterogeneities in lipids and proteins." Physical Chemistry Chemical Physics 17, no. 24 (2015): 15598-15607.en_US
dc.identifier.doi10.1039/c4cp05877a
dc.identifier.urihttp://hdl.handle.net/2152/39103
dc.language.isoengen_US
dc.publisherPhysical Chemistry Chemical Physicsen_US
dc.relation.ispartofUT Faculty/Researcher Worksen_US
dc.rights.restrictionOpenen_US
dc.subjectmembrane adhesionen_US
dc.titleSpecific Adhesion of Membranes Simultaneously Supports Dual Heterogeneities in Lipids and Proteinsen_US
dc.typeArticleen_US

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