Cargo Transport by Cytoplasmic Dynein Can Center Embryonic Centrosomes

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dc.creatorLongoria, Rafael A.en
dc.creatorShubeita, Georgeen
dc.date.accessioned2014-12-15T17:10:51Zen
dc.date.available2014-12-15T17:10:51Zen
dc.date.issued2013-07-01en
dc.descriptionen
dc.description.abstractTo complete meiosis II in animal cells, the male DNA material needs to meet the female DNA material contained in the female pronucleus at the egg center, but it is not known how the male pronucleus, deposited by the sperm at the periphery of the cell, finds the cell center in large eggs. Pronucleus centering is an active process that appears to involve microtubules and molecular motors. For small and medium-sized cells, the force required to move the centrosome can arise from either microtubule pushing on the cortex, or cortically-attached dynein pulling on microtubules. However, in large cells, such as the fertilized Xenopus laevis embryo, where microtubules are too long to support pushing forces or they do not reach all boundaries before centrosome centering begins, a different force generating mechanism must exist. Here, we present a centrosome positioning model in which the cytosolic drag experienced by cargoes hauled by cytoplasmic dynein on the sperm aster microtubules can move the centrosome towards the cell’s center. We find that small, fast cargoes (diameter ~100 nm, cargo velocity ~2 µm/s) are sufficient to move the centrosome in the geometry of the Xenopus laevis embryo within the experimentally observed length and time scales.en
dc.description.catalogingnoteEmail: Shubeita@chaos.utexas.eduen
dc.description.departmentPhysicsen
dc.description.departmentCellular and Molecular Biologyen
dc.description.departmenten
dc.description.sponsorshipThis work was supported in part by the National Science Foundation grant PHY-0957811 to GTS. (www.nsf.gov) The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. No additional external funding was received for this study.en
dc.identifier.Filenamejournal.pone.0067710.pdfen
dc.identifier.citationLongoria RA, Shubeita GT (2013) Cargo Transport by Cytoplasmic Dynein Can Center Embryonic Centrosomes. PLoS ONE 8(7): e67710. doi:10.1371/journal.pone.0067710en
dc.identifier.doiDOI: 10.1371/journal.pone.0067710en
dc.identifier.urihttp://hdl.handle.net/2152/27942en
dc.language.isoEnglishen
dc.publisherPLOS Oneen
dc.rightsAdministrative deposit of works to UT Digital Repository: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access at http://www.plosone.org. The public license is specified as CC-BY: http://creativecommons.org/licenses/by/4.0/. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en
dc.subjectcentrosomesen
dc.subjectdragen
dc.subjectmicrotubule motorsen
dc.subjectmicrotubulesen
dc.subjectmolecular motorsen
dc.subjectspermen
dc.subjectvesiclesen
dc.subjectviscosityen
dc.titleCargo Transport by Cytoplasmic Dynein Can Center Embryonic Centrosomesen
dc.typeArticleen

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