Transforming a Pair of Orthogonal tRNA-aminoacyl-tRNA Synthetase from Archaea to Function in Mammalian Cells
| dc.creator | Thibodeaux, Gabrielle Nina | en |
| dc.creator | Liang, Xiang | en |
| dc.creator | Moncivais, Kathryn | en |
| dc.creator | Umeda, Aiko | en |
| dc.creator | Singer, Oded | en |
| dc.creator | Alfonta, Lital | en |
| dc.creator | Zhang, Zhiwen Jonathan | en |
| dc.date.accessioned | 2013-06-28T14:42:15Z | en |
| dc.date.available | 2013-06-28T14:42:15Z | en |
| dc.date.issued | 2010-06-22 | en |
| dc.description | Gabrielle Nina Thibodeaux is with UT Austin, Xiang Liang is with UT Austin, Kathryn Moncivais is with UT Austin, Aiko Umeda is with UT Austin, Oded Singer is with The Salk Institute for Biological Studies, Lital Alfonta is with Ben-Gurion University of the Negev, Zhiwen Jonathan Zhang is with UT Austin. | en |
| dc.description.abstract | A previously engineered Methanocaldococcus jannaschii –tyrosyl-tRNA synthetase pair orthogonal to Escherichia coli was modified to become orthogonal in mammalian cells. The resulting -tyrosyl-tRNA synthetase pair was able to suppress an amber codon in the green fluorescent protein, GFP, and in a foldon protein in mammalian cells. The methodology reported here will allow rapid transformation of the much larger collection of existing tyrosyl-tRNA synthetases that were already evolved for the incorporation of an array of over 50 unnatural amino acids into proteins in Escherichia coli into proteins in mammalian cells. Thus we will be able to introduce a large array of possibilities for protein modifications in mammalian cells. | en |
| dc.description.department | Pharmaceutical Sciences | en |
| dc.description.sponsorship | L.A. acknowledges the Edmond J. Safra Center for the Design and Engineering of Functional Biopolymers for funding this research, (URL of Funding Agency: http://www.edmondjsafra.org/). Z.Z. acknowledges a Welch Foundation Research Grant (F1618) and NIH R01 CA120168. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | en |
| dc.identifier.citation | Thibodeaux GN, Liang X, Moncivais K, Umeda A, Singer O, et al. (2010) Transforming a Pair of Orthogonal tRNA-aminoacyl-tRNA Synthetase from Archaea to Function in Mammalian Cells. PLoS ONE 5(6): e11263. doi:10.1371/journal.pone.0011263 | en |
| dc.identifier.doi | 10.1371/journal.pone.0011263 | en |
| dc.identifier.uri | http://hdl.handle.net/2152/20493 | en |
| dc.language.iso | eng | en |
| dc.publisher | Public Library of Science | en |
| dc.rights | Attribution 3.0 United States | en |
| dc.rights | CC-BY | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | en |
| dc.subject | 293T cells | en |
| dc.subject | Codons | en |
| dc.subject | Engineering and technology | en |
| dc.subject | Green fluorescent protein | en |
| dc.subject | Plasmid construction | en |
| dc.subject | Polymerase chain reaction amplification | en |
| dc.subject | Protein expression | en |
| dc.subject | Transfer RNA | en |
| dc.title | Transforming a Pair of Orthogonal tRNA-aminoacyl-tRNA Synthetase from Archaea to Function in Mammalian Cells | en |
| dc.type | Article | en |