Tgif1 Counterbalances The Activity Of Core Pluripotency Factors In Mouse Embryonic Stem Cells

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dc.contributor.utaustinauthorLee, Bum-Kyuen_US
dc.contributor.utaustinauthorShen, Wenwenen_US
dc.contributor.utaustinauthorLee, Jiwoonen_US
dc.contributor.utaustinauthorRhee, Catherineen_US
dc.contributor.utaustinauthorChung, Haewonen_US
dc.contributor.utaustinauthorKim, Jonghwanen_US
dc.creatorLee, Bum-Kyuen_US
dc.creatorShen, Wenwenen_US
dc.creatorLee, Jiwoonen_US
dc.creatorRhee, Catherineen_US
dc.creatorChung, Haewonen_US
dc.creatorKim, Kun-Yongen_US
dc.creatorPark, In-Hyunen_US
dc.creatorKim, Jonghwanen_US
dc.date.accessioned2016-10-28T19:52:05Z
dc.date.available2016-10-28T19:52:05Z
dc.date.issued2015-10en_US
dc.description.abstractCore pluripotency factors, such as Oct4, Sox2, and Nanog, play important roles in maintaining embryonic stem cell (ESC) identity by autoregulatory feedforward loops. Nevertheless, the mechanism that provides precise control of the levels of the ESC core factors without indefinite amplification has remained elusive. Here, we report the direct repression of core pluripotency factors by Tgif1, a previously known terminal repressor of TGF beta/activin/nodal signaling. Overexpression of Tgif1 reduces the levels of ESC core factors, whereas its depletion leads to the induction of the pluripotency factors. We confirm the existence of physical associations between Tgif1 and Oct4, Nanog, and HDAC1/2 and further show the level of Tgif1 is not significantly altered by treatment with an activator/inhibitor of the TGF beta/activin/nodal signaling. Collectively, our findings establish Tgif1 as an integral member of the core regulatory circuitry of mouse ESCs that counterbalances the levels of the core pluripotency factors in a TGF beta/activin/nodal-independent manner.en_US
dc.description.departmentMolecular Biosciencesen_US
dc.description.sponsorshipCancer Prevention Research Institute of Texas (CPRIT) R1106en_US
dc.identifierdoi:10.15781/T23J39392
dc.identifier.citationLee, Bum-Kyu, Wenwen Shen, Jiwoon Lee, Catherine Rhee, Haewon Chung, Kun-Yong Kim, In-Hyun Park, and Jonghwan Kim. "Tgif1 Counterbalances the Activity of Core Pluripotency Factors in Mouse Embryonic Stem Cells." Cell report, Vol. 13, No. 1 (Oct., 2015): 52-60.en_US
dc.identifier.doi10.1016/j.celrep.2015.08.067en_US
dc.identifier.issn2211-1247en_US
dc.identifier.urihttp://hdl.handle.net/2152/43276
dc.language.isoEnglishen_US
dc.relation.ispartofen_US
dc.relation.ispartofserialCell Reportsen_US
dc.rightsAdministrative deposit of works to Texas ScholarWorks: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access or the publisher allows a PDF version of the article to be freely posted online. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en_US
dc.rights.restrictionOpenen_US
dc.subjectsmad transcriptional corepressoren_US
dc.subjectgeneen_US
dc.subjectdifferentiationen_US
dc.subjectexpressionen_US
dc.subjectnetworken_US
dc.subjectfibroblastsen_US
dc.subjectrepressionen_US
dc.subjectcircuitryen_US
dc.subjectmodesen_US
dc.subjectstateen_US
dc.subjectcell biologyen_US
dc.titleTgif1 Counterbalances The Activity Of Core Pluripotency Factors In Mouse Embryonic Stem Cellsen_US
dc.typeArticleen_US

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