Replicase-Based Plasmid DNA Shows Anti-Tumor Activity

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dc.contributor.utaustinauthorRodriguez, B. Leticiaen_US
dc.contributor.utaustinauthorChung, Woon-Gyeen_US
dc.contributor.utaustinauthorCui, Zhengrongen_US
dc.creatorRodriguez, B. Leticiaen_US
dc.creatorYu, Zhenen_US
dc.creatorChung, Woon-Gyeen_US
dc.creatorWeiss, Richarden_US
dc.creatorCui, Zhengrongen_US
dc.date.accessioned2016-10-28T19:49:13Z
dc.date.available2016-10-28T19:49:13Z
dc.date.issued2011-03en_US
dc.description.abstractDouble stranded RNA (dsRNA) has multiple anti-tumor mechanisms. Over the past several decades, there have been numerous attempts to utilize synthetic dsRNA to control tumor growth in animal models and clinical trials. Recently, it became clear that intracellular dsRNA is more effective than extracellular dsRNA on promoting apoptosis and orchestrating adaptive immune responses. To overcome the difficulty in delivering a large dose of synthetic dsRNA into tumors, we propose to deliver a RNA replicase-based plasmid DNA, hypothesizing that the dsRNA generated by the replicase-based plasmid in tumor cells will inhibit tumor growth. Methods: The anti-tumor activity of a plasmid (pSIN-beta) that encodes the sindbis RNA replicase genes (nsp1-4) was evaluated in mice with model tumors (TC-1 lung cancer cells or B16 melanoma cells) and compared to a traditional pCMV-beta plasmid. Results: In cell culture, transfection of tumor cells with pSIN-beta generated dsRNA. In mice with model tumors, pSIN-beta more effectively delayed tumor growth than pCMV-beta, and in some cases, eradicated the tumors. Conclusion: RNA replicase-based plasmid may be exploited to generate intracellular dsRNA to control tumor growth.en_US
dc.description.departmentPharmaceutical Sciencesen_US
dc.description.sponsorshipNational Cancer Institute CA135274, CA135274-S1en_US
dc.identifierdoi:10.15781/T22B8VF3S
dc.identifier.citationRodriguez, B. Leticia, Zhen Yu, Woon-Gye Chung, Richard Weiss, and Zhengrong Cui. "Replicase-based plasmid DNA shows anti-tumor activity." BMC cancer, Vol. 11, No. 1 (Mar., 2011): 1.en_US
dc.identifier.doi10.1186/1471-2407-11-110en_US
dc.identifier.issn1471-2407en_US
dc.identifier.urihttp://hdl.handle.net/2152/43137
dc.language.isoEnglishen_US
dc.relation.ispartofen_US
dc.relation.ispartofserialBMC Canceren_US
dc.rightsAdministrative deposit of works to Texas ScholarWorks: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access or the publisher allows a PDF version of the article to be freely posted online. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en_US
dc.rights.restrictionOpenen_US
dc.subjectdouble-stranded-rnaen_US
dc.subjectpolycytidylic acid/cationic liposomeen_US
dc.subjecttoll-likeen_US
dc.subjectreceptor-3en_US
dc.subjectsindbis-virus nsp4en_US
dc.subjectsynthetic dsrnaen_US
dc.subjectinterferon inductionen_US
dc.subjecttumorsen_US
dc.subjectvaccinesen_US
dc.subjectcanceren_US
dc.subjectresponsesen_US
dc.subjectoncologyen_US
dc.titleReplicase-Based Plasmid DNA Shows Anti-Tumor Activityen_US
dc.typeArticleen_US

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