Epigenetic Control of Gonadal Aromatase (cyp19a1) in Temperature-Dependent Sex Determination of Red-Eared Slider Turtles

Simple item page
dc.creatorMatsumoto, Yuikoen
dc.creatorBuemio, Alvinen
dc.creatorChu, Randyen
dc.creatorVafaee, Mozhgonen
dc.creatorCrews, Daviden
dc.date.accessioned2014-12-15T17:09:45Zen
dc.date.available2014-12-15T17:09:45Zen
dc.date.issued2013-06-07en
dc.description.abstractIn the red-eared slider turtle (Trachemys scripta), a species with temperature-dependent sex determination (TSD), the expression of the aromatase gene during gonad development is strictly limited to the female-producing temperature. The underlying mechanism remains unknown. In this study, we identified the upstream 5′-flanking region of the aromatase gene, gonad-specific promoter, and the temperature-dependent DNA methylation signatures during gonad development in the red-eared slider turtle. The 5′-flanking region of the slider aromatase exhibited sequence similarities to the aromatase genes of the American alligator, chicken, quail, and zebra finch. A putative TATA box was located 31 bp upstream of the gonad-specific transcription start site. DNA methylation at the CpG sites between the putative binding sites of the fork head domain factor (FOX) and vertebrate steroidogenic factor 1 (SF1) and adjacent TATA box in the promoter region were significantly lower in embryonic gonads at the female-producing temperature compared the male-producing temperature. A shift from male- to female-, but not from female- to male-, producing temperature changed the level of DNA methylation in gonads. Taken together these results indicate that the temperature, particularly female-producing temperature, allows demethylation at the specific CpG sites of the promoter region which leads the temperature-specific expression of aromatase during gonad development.en
dc.description.catalogingnoteEmail: crews@austin.utexas.eduen
dc.description.departmentIntegrative Biologyen
dc.description.sponsorshipThis work was supported by the National Science Foundation (grant number IOS-1051623). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.identifier.Filenamejournal.pone.0063599.pdfen
dc.identifier.citationMatsumoto Y, Buemio A, Chu R, Vafaee M, Crews D (2013) Epigenetic Control of Gonadal Aromatase (cyp19a1) in Temperature-Dependent Sex Determination of Red-Eared Slider Turtles. PLoS ONE 8(6): e63599. doi:10.1371/journal.pone.0063599en
dc.identifier.doiDOI: 10.1371/journal.pone.0063599en
dc.identifier.urihttp://hdl.handle.net/2152/27791en
dc.language.isoEnglishen
dc.publisherPLOS Oneen
dc.rightsAdministrative deposit of works to UT Digital Repository: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access at http://www.plosone.org. The public license is specified as CC-BY: http://creativecommons.org/licenses/by/4.0/. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en
dc.subjectDNA methylationen
dc.subjectDNA sequenceen
dc.subjectDNA transcriptionen
dc.subjectgene expressionen
dc.subjectgonadsen
dc.subjectpolymerase chain reactionen
dc.subjecttranscription factorsen
dc.subjectturtlesen
dc.titleEpigenetic Control of Gonadal Aromatase (cyp19a1) in Temperature-Dependent Sex Determination of Red-Eared Slider Turtlesen
dc.typeArticleen

Access full-text files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
journal.pone.0063599.pdf
Size:
2.15 MB
Format:
Adobe Portable Document Format
Download

Collections

UT Faculty/Researcher Works