Insights into the Evolution of Mammalian Telomerase: Platypus TERT Shares Similarities with Genes of Birds and Other Reptiles and Localizes on Sex Chromosomes

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dc.contributor.utaustinauthorHrdlickova, Radmilaen_US
dc.contributor.utaustinauthorNehyba, Jirien_US
dc.contributor.utaustinauthorBose, Henry R.en_US
dc.creatorHrdlickova, Radmilaen_US
dc.creatorNehyba, Jirien_US
dc.creatorLim, Shu Lyen_US
dc.creatorGrutzner, Franken_US
dc.creatorBose, Henry R.en_US
dc.date.accessioned2016-10-28T19:49:46Z
dc.date.available2016-10-28T19:49:46Z
dc.date.issued2012-06en_US
dc.description.abstractThe TERT gene encodes the catalytic subunit of the telomerase complex and is responsible for maintaining telomere length. Vertebrate telomerase has been studied in eutherian mammals, fish, and the chicken, but less attention has been paid to other vertebrates. The platypus occupies an important evolutionary position, providing unique insight into the evolution of mammalian genes. We report the cloning of a platypus TERT (OanTERT) ortholog, and provide a comparison with genes of other vertebrates. Results: The OanTERT encodes a protein with a high sequence similarity to marsupial TERT and avian TERT. Like the TERT of sauropsids and marsupials, as well as that of sharks and echinoderms, OanTERT contains extended variable linkers in the N-terminal region suggesting that they were present already in basal vertebrates and lost independently in ray-finned fish and eutherian mammals. Several alternatively spliced OanTERT variants structurally similar to avian TERT variants were identified. Telomerase activity is expressed in all platypus tissues like that of cold-blooded animals and murine rodents. OanTERT was localized on pseudoautosomal regions of sex chromosomes X3/Y2, expanding the homology between human chromosome 5 and platypus sex chromosomes. Synteny analysis suggests that TERT co-localized with sex-linked genes in the last common mammalian ancestor. Interestingly, female platypuses express higher levels of telomerase in heart and liver tissues than do males. Conclusions: OanTERT shares many features with TERT of the reptilian outgroup, suggesting that OanTERT represents the ancestral mammalian TERT. Features specific to TERT of eutherian mammals have, therefore, evolved more recently after the divergence of monotremes.en_US
dc.description.departmentCellular and Molecular Biologyen_US
dc.description.sponsorshipNational Cancer Institute CA33192, CA098151en_US
dc.identifierdoi:10.15781/T2PZ51P82
dc.identifier.citationHrdli?ková, Radmila, Ji?í Nehyba, Shu Ly Lim, Frank Grützner, and Henry R. Bose. "Insights into the evolution of mammalian telomerase: Platypus TERT shares similarities with genes of birds and other reptiles and localizes on sex chromosomes." BMC genomics, Vol. 13, No. 1 (Jun., 2012): 216.en_US
dc.identifier.doi10.1186/1471-2164-13-216en_US
dc.identifier.issn1471-2164en_US
dc.identifier.urihttp://hdl.handle.net/2152/43163
dc.language.isoEnglishen_US
dc.relation.ispartofen_US
dc.relation.ispartofserialBMC Genomicsen_US
dc.rightsAdministrative deposit of works to Texas ScholarWorks: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access or the publisher allows a PDF version of the article to be freely posted online. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en_US
dc.rights.restrictionOpenen_US
dc.subjectplatypusen_US
dc.subjectterten_US
dc.subjecttelomeraseen_US
dc.subjectalternative splicingen_US
dc.subjecttelomeresen_US
dc.subjectsexen_US
dc.subjectchromosomesen_US
dc.subjectreverse-transcriptase componenten_US
dc.subjectcatalytic subunit geneen_US
dc.subjectin-vivoen_US
dc.subjectornithorhynchus-anatinusen_US
dc.subject(ttaggg)(n) sequencesen_US
dc.subjectmolecular-cloningen_US
dc.subjectmouse telomeraseen_US
dc.subjectlife-spanen_US
dc.subjectexpressionen_US
dc.subjectlengthen_US
dc.subjectbiotechnology & applied microbiologyen_US
dc.subjectgenetics & heredityen_US
dc.titleInsights into the Evolution of Mammalian Telomerase: Platypus TERT Shares Similarities with Genes of Birds and Other Reptiles and Localizes on Sex Chromosomesen_US
dc.typeArticleen_US

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