Targeted Enrichment: Maximizing Orthologous Gene Comparisons across Deep Evolutionary Time

Simple item page
dc.creatorHedtke, Shannon M.en
dc.creatorMorgan, Matthew J.en
dc.creatorCannatella, David C.en
dc.creatorHillis, David M.en
dc.date.accessioned2014-12-15T17:10:47Zen
dc.date.available2014-12-15T17:10:47Zen
dc.date.issued2013-07-02en
dc.descriptionen
dc.description.abstractEstimated phylogenies of evolutionarily diverse taxa will be well supported and more likely to be historically accurate when the analysis contains large amounts of data–many genes sequenced across many taxa. Inferring such phylogenies for non-model organisms is challenging given limited resources for whole-genome sequencing. We take advantage of genomic data from a single species to test the limits of hybridization-based enrichment of hundreds of exons across frog species that diverged up to 250 million years ago. Enrichment success for a given species depends greatly on the divergence time between it and the reference species, and the resulting alignment contains a significant proportion of missing data. However, our alignment generates a well-supported phylogeny of frogs, suggesting that this technique is a practical solution towards resolving relationships across deep evolutionary time.en
dc.description.catalogingnoteEmail: shannon.hedtke@gmail.comen
dc.description.departmentCenter for Computational Biology and Bioinformaticsen
dc.description.departmentTexas Natural Science Centeren
dc.description.sponsorshipThis work was funded by a National Science Foundation Early-concept Grant for Exploratory Research, DEB 0942956. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.identifier.Filenamejournal.pone.0067908.pdfen
dc.identifier.citationHedtke SM, Morgan MJ, Cannatella DC, Hillis DM (2013) Targeted Enrichment: Maximizing Orthologous Gene Comparisons across Deep Evolutionary Time. PLoS ONE 8(7): e67908. doi:10.1371/journal.pone.0067908en
dc.identifier.doiDOI: 10.1371/journal.pone.0067908en
dc.identifier.urihttp://hdl.handle.net/2152/27931en
dc.language.isoEnglishen
dc.publisherPLOS Oneen
dc.rightsAdministrative deposit of works to UT Digital Repository: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access at http://www.plosone.org. The public license is specified as CC-BY: http://creativecommons.org/licenses/by/4.0/. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en
dc.subjectAmphibian genomicsen
dc.subjectDNA sequencesen
dc.subjectfrogsen
dc.subjectgenome evolutionen
dc.subjectgenommic librariesen
dc.subjectphylogenetic analysisen
dc.subjectphylogeneticsen
dc.subjectxenopusen
dc.titleTargeted Enrichment: Maximizing Orthologous Gene Comparisons across Deep Evolutionary Timeen
dc.typeArticleen

Access full-text files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
journal.pone.0067908.pdf
Size:
457.71 KB
Format:
Adobe Portable Document Format
Download

Collections

UT Faculty/Researcher Works