Rapid Targeted Gene Disruption in Bacillus Anthracis

Simple item page
dc.contributor.utaustinauthorPerutka, Jirien_US
dc.contributor.utaustinauthorWhitt, Jacob T.en_US
dc.contributor.utaustinauthorEllington, Andrewen_US
dc.contributor.utaustinauthorLambowitz, Alan M.en_US
dc.creatorSaldanha, Roland J.en_US
dc.creatorPemberton, Adinen_US
dc.creatorShiflett, Patricken_US
dc.creatorPerutka, Jirien_US
dc.creatorWhitt, Jacob T.en_US
dc.creatorEllington, Andrewen_US
dc.creatorLambowitz, Alan M.en_US
dc.creatorKramer, Ryanen_US
dc.creatorTaylor, Deborahen_US
dc.creatorLamkin, Thomas J.en_US
dc.date.accessioned2016-10-28T19:53:54Z
dc.date.available2016-10-28T19:53:54Z
dc.date.issued2013-09en_US
dc.description.abstractAnthrax is a zoonotic disease recognized to affect herbivores since Biblical times and has the widest range of susceptible host species of any known pathogen. The ease with which the bacterium can be weaponized and its recent deliberate use as an agent of terror, have highlighted the importance of gaining a deeper understanding and effective countermeasures for this important pathogen. High quality sequence data has opened the possibility of systematic dissection of how genes distributed on both the bacterial chromosome and associated plasmids have made it such a successful pathogen. However, low transformation efficiency and relatively few genetic tools for chromosomal manipulation have hampered full interrogation of its genome. Results: Group II introns have been developed into an efficient tool for site-specific gene inactivation in several organisms. We have adapted group II intron targeting technology for application in Bacillus anthracis and generated vectors that permit gene inactivation through group II intron insertion. The vectors developed permit screening for the desired insertion through PCR or direct selection of intron insertions using a selection scheme that activates a kanamycin resistance marker upon successful intron insertion. Conclusions: The design and vector construction described here provides a useful tool for high throughput experimental interrogation of the Bacillus anthracis genome and will benefit efforts to develop improved vaccines and therapeutics.en_US
dc.description.departmentCellular and Molecular Biologyen_US
dc.description.sponsorshipChem-Bio Diagnostics program from the Department of Defense Chemical and Biological Defense program through the Defense Threat Reduction Agency (DTRA) B102387Men_US
dc.description.sponsorshipNIH GM037949en_US
dc.description.sponsorshipWelch Foundation F-1607en_US
dc.identifierdoi:10.15781/T2BN9X57X
dc.identifier.citationSaldanha, Roland J., Adin Pemberton, Patrick Shiflett, Jiri Perutka, Jacob T. Whitt, Andrew Ellington, Alan M. Lambowitz, Ryan Kramer, Deborah Taylor, and Thomas J. Lamkin. "Rapid targeted gene disruption in Bacillus anthracis." BMC biotechnology, Vol. 13, No. 1 (Sep., 2013): 72.en_US
dc.identifier.doi10.1186/1472-6750-13-72en_US
dc.identifier.issn1472-6750en_US
dc.identifier.urihttp://hdl.handle.net/2152/43367
dc.language.isoEnglishen_US
dc.relation.ispartofen_US
dc.relation.ispartofserialBMC Biotechnologyen_US
dc.rightsAdministrative deposit of works to Texas ScholarWorks: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access or the publisher allows a PDF version of the article to be freely posted online. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en_US
dc.rights.restrictionOpenen_US
dc.subjectgroup-ii intronen_US
dc.subjectescherichia-colien_US
dc.subjectgenome sequenceen_US
dc.subjectdnaen_US
dc.subjectendonucleaseen_US
dc.subjectbacteriaen_US
dc.subjectmobilityen_US
dc.subjectproteinen_US
dc.subjectrnaen_US
dc.subjecttranscriptionen_US
dc.subjectbiotechnology & applied microbiologyen_US
dc.titleRapid Targeted Gene Disruption in Bacillus Anthracisen_US
dc.typeArticleen_US

Access full-text files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
2013_09_Saldanha.pdf
Size:
785.56 KB
Format:
Adobe Portable Document Format
Download

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.65 KB
Format:
Plain Text
Description:
Download

Collections

UT Faculty/Researcher Works