Crystallization And Preliminary X-Ray Analysis Of A Chitinase From The Fungal Pathogen Coccidioides Immitis

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dc.contributor.utaustinauthorHollis, Thomasen
dc.contributor.utaustinauthorMonzingo, Arthur F.en
dc.contributor.utaustinauthorBortone, Karaen
dc.contributor.utaustinauthorSchelp, Elisabethen
dc.contributor.utaustinauthorCox, Rebeccaen
dc.contributor.utaustinauthorRobertus, Jon D.en
dc.creatorHollis, Thomasen
dc.creatorMonzingo, Arthur F.en
dc.creatorBortone, Karaen
dc.creatorSchelp, Elisabethen
dc.creatorCox, Rebeccaen
dc.creatorRobertus, Jon D.en
dc.date.accessioned2015-09-09T15:50:30Zen
dc.date.available2015-09-09T15:50:30Zen
dc.date.issued1998-11en
dc.description.abstractChitinase is necessary for fungal growth and cell division and, therefore, is an ideal target for the design of inhibitors which may act as antifungal agents. A chitinase from the fungal pathogen Coccidioides immitis has been expressed as a fusion protein with gluathione-S-transferase (GST), which aids in purification. After cleavage from GST, chitinase was crystallized from 30% PEG 4000 in 0.1 M sodium acetate pH 4.6. The crystals have a tetragonal crystal lattice and belong to space group P4(1)2(1)2 or P4(3)2(1)2 and diffract to 2.2 Angstrom resolution. The unit-cell parameters are a = b = 91.2, c = 95.4 Angstrom; there is only one chitinase molecule in the asymmetric unit.en
dc.description.departmentMolecular Biosciencesen
dc.description.sponsorshipNational Institutes of Health GM 30048en
dc.description.sponsorshipNational Science Foundation MCB-9601096en
dc.description.sponsorshipFoundation for Researchen
dc.description.sponsorshipWelch Foundationen
dc.identifier.citationHollis, Thomas, Monzingo, Arthur F., Bortone, Kara, Schelp, Elisabeth, Cox, Rebecca, Robertus, Jon D., >Crystallization and preliminary X-ray analysis of a chitinase from the fungal pathogen Coccidioides immitis, Acta Crystallogr D Biol Crystallogr. 1998 Nov 1;54(Pt 6 Pt 2):1412-3. doi: 10.1107/S0907444998008531en
dc.identifier.doi10.1107/s0907444998008531en
dc.identifier.issn0907-4449en
dc.identifier.urihttp://hdl.handle.net/2152/31094en
dc.identifier.urlen
dc.language.isoEnglishen
dc.relation.ispartofserialActa Crystallographica Section D-Biological Crystallographyen
dc.rightsAdministrative deposit of works to Texas ScholarWorks: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access or the publisher allows a PDF version of the article to be freely posted online. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en
dc.rights.holderen
dc.subjectcomplement-fixation antigenen
dc.subjectbiochemical research methodsen
dc.subjectbiochemistry & molecular biologyen
dc.subjectbiophysicsen
dc.subjectcrystallographyen
dc.titleCrystallization And Preliminary X-Ray Analysis Of A Chitinase From The Fungal Pathogen Coccidioides Immitisen
dc.typeArticleen

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