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dc.creatorSabree, Zakee L.en
dc.creatorMoran, Nancy A.en
dc.date.accessioned2014-12-15T17:10:52Zen
dc.date.available2014-12-15T17:10:52Zen
dc.date.issued2014-03-11en
dc.identifier.citationSabree, Zakee L., and Nancy A. Moran. “Host-Specific Assemblages Typify Gut Microbial Communities of Related Insect Species.” SpringerPlus 3, no. 1 (March 11, 2014): 138. doi:10.1186/2193-1801-3-138.en
dc.identifier.urihttp://hdl.handle.net/2152/27945en
dc.descriptionZakee L Sabree12* and Nancy A Moran13 Author Affiliations 1 Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT 06511, USA 2 Department of Evolution, Ecology and Organismal Biology, The Ohio State University, Columbus, OH 43210, USA 3 Section of Integrative Biology, The University of Texas at Austin, Austin, TX 78712, USAen
dc.description.abstractMutualisms between microbes and insects are ubiquitous and facilitate exploitation of various trophic niches by host insects. Dictyopterans (mantids, cockroaches and termites) exhibit trophisms that range from omnivory to strict wood-feeding and maintain beneficial symbioses with the obligate endosymbiont, Blattabacterium, and/or diverse gut microbiomes that include cellulolytic and diazotrophic microbes. While Blattabacterium in omnivorous Periplaneta is fully capable of provisioning essential amino acids, in wood-feeding dictyopterans it has lost many genes for their biosynthesis (Mastotermes and Cryptocercus) or is completely absent (Heterotermes). The conspicuous functional degradation and absence of Blattabacterium in most strict wood-feeding dictyopteran insects suggest that alternative means of acquiring nutrients limited in their diet are being employed. A 16S rRNA gene amplicon resequencing approach was used to deeply sample the composition and diversity of gut communities in related dictyopteran insects to explore the possibility of shifts in symbiont allegiances during termite and cockroach evolution. The gut microbiome of Periplaneta, which has a fully functional Blattabacterium, exhibited the greatest within-sample operational taxonomic unit (OTU) diversity and abundance variability than those of Mastotermes and Cryptocercus, whose Blattabacterium have shrunken genomes and reduced nutrient provisioning capabilities. Heterotermes lacks Blattabacterium and a single OTU that was 95% identical to a Bacteroidia-assigned diazotrophic endosymbiont of an anaerobic cellulolytic protist termite gut inhabitant samples consistently dominates its gut microbiome. Many host-specific OTUs were identified in all host genera, some of which had not been previously detected, indicating that deep sampling by pyrotag sequencing has revealed new taxa that remain to be functionally characterized. Further analysis is required to uncover how consistently detected taxa in the cockroach and termite gut microbiomes, as well as the total community, contribute to host diet choice and impact the fate of Blattabacterium in dictyopterans.en
dc.description.sponsorshipen
dc.language.isoEnglishen
dc.publisherSpringer Plusen
dc.rightsAdministrative deposit of works to UT Digital Repository: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access at http://www.biomedcentral.com. The public license is specified as CC-BY: http://creativecommons.org/licenses/by/4.0/. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en
dc.subjectcockroachesen
dc.subjecttermitesen
dc.subjectgut microbiotaen
dc.subjectBlattabacteriumen
dc.titleHost-specific assemblages typify gut microbial communities of related insect speciesen
dc.typeArticleen
dc.description.departmentIntegrative Biologyen
dc.description.catalogingnotesabree.8@osu.eduen
dc.identifier.Filename2193-1801-3-138.pdfen
dc.identifier.doidoi:10.1186/2193-1801-3-138en


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