In vivo optical imaging to investigate neurovascular structure and cerebral hemodynamics

Simple item page
dc.contributor.advisorDunn, Andrew Kenneth, 1970-
dc.contributor.committeeMemberMilner, Thomas E
dc.contributor.committeeMemberYeh, Hsin-Chih "Tim"
dc.contributor.committeeMemberJones, Theresa A
dc.creatorMiller, David Roger
dc.creator.orcid0000-0002-6210-7941
dc.date.accessioned2018-08-21T16:44:24Z
dc.date.available2018-08-21T16:44:24Z
dc.date.created2018-05
dc.date.issued2018-05-01
dc.date.submittedMay 2018
dc.date.updated2018-08-21T16:44:24Z
dc.description.abstractThe ability to visualize structural features of the brain and associated functional information has fueled a revolution in our understanding of the brain. The optical technique two-photon microscopy (2PM) is widely used to study individual neural circuits and blood vessel networks in vivo because it is minimally invasive and provides three-dimensional images with cellular resolution. There is rising interest from neuroscientists for the ability to extend the traditional imaging depth of 2PM, which is typically limited to ∼500 μm below the surface of the brain. In this dissertation, I detail the development of a novel laser source that enables deep-tissue in vivo multiphoton microscopy imaging of blood vessel networks and neurons. Using an excitation wavelength near 1,300 nm at which scattering in tissue is minimized, I demonstrate the ability to chronically study vascular morphology and dynamics as well as neuron morphology at imaging depths of 1 mm and beyond.
dc.description.departmentBiomedical Engineering
dc.format.mimetypeapplication/pdf
dc.identifierdoi:10.15781/T2VX06N42
dc.identifier.urihttp://hdl.handle.net/2152/68100
dc.language.isoen
dc.subjectTwo-photon microscopy
dc.subjectThree-photon microscopy
dc.subjectDeep-tissue brain imaging
dc.subjectOptical parametric amplifier
dc.titleIn vivo optical imaging to investigate neurovascular structure and cerebral hemodynamics
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentBiomedical Engineering
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorThe University of Texas at Austin
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy

Access full-text files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
MILLER-DISSERTATION-2018.pdf
Size:
9.64 MB
Format:
Adobe Portable Document Format
Download

License bundle

Now showing 1 - 2 of 2
No Thumbnail Available
Name:
PROQUEST_LICENSE.txt
Size:
4.45 KB
Format:
Plain Text
Description:
Download
No Thumbnail Available
Name:
LICENSE.txt
Size:
1.84 KB
Format:
Plain Text
Description:
Download

Collections

UT Electronic Theses and Dissertations