Stability of C-Reactive Protein in saliva using an ELISA test
| dc.contributor.advisor | John McDevitt | en |
| dc.creator | Peddaiahgari, Rajesh | en |
| dc.date.accessioned | 2011-09-01T20:11:24Z | en |
| dc.date.available | 2011-09-01T20:11:24Z | en |
| dc.date.issued | 2008 | en |
| dc.description.abstract | This project was done in order to measure the stability of proteins in general; in particular C-Reactive Protein (CRP) which is very important in cardiovascular studies. Current methods of handling CRP is to freeze the sample at -80°C for long term use, or -20°C for medium term use, or 4°C for short term use. This experiment was done in order to measure how much CRP will degrade after a certain time with different matrices. From this project, no conclusive data was gathered to give a certain approximation on the stability of CRP. The data that was gathered did tell us that CRP is most stable with PBS with 0.5% Albumin (PBSA) than saliva, which is more stable than in nanopure water. Furthermore, the data showed that samples kept at -20°C were more stable than those kept at 4°C. | en |
| dc.description.department | Biochemistry | en |
| dc.identifier.uri | http://hdl.handle.net/2152/13367 | en |
| dc.language.iso | eng | en |
| dc.subject | College of Natural Sciences | en |
| dc.subject | proteins | en |
| dc.subject | C-Reactive Protein | en |
| dc.subject | saliva | en |
| dc.subject | ELISA | en |
| dc.title | Stability of C-Reactive Protein in saliva using an ELISA test | en |
| dc.type | Thesis | en |