A miniaturized probe for combined two-photon microscopy and femtosecond laser microsurgery

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dc.contributor.advisorBen-Yakar, Adela
dc.creatorHoy, Christopher Luk, 1982-
dc.date.accessioned2017-03-16T14:03:16Z
dc.date.available2017-03-16T14:03:16Z
dc.date.issued2007-12
dc.description.abstractThis thesis presents the design, fabrication, and characterization of a miniaturized two-photon fluorescence microscope system. The system is designed to act as a first step towards the design of a two-photon endoscope system capable of in vivo diagnostic imaging and delivery of high energy femtosecond laser pulses for microsurgery. Optical modeling software (TracePro®) was used to design a compact (10 × 15 × 40 mm³) microscope system where field of view (FOV), resolution, and collection efficiency were identified as the key areas of performance. The resulting design utilizes two-axis gimbaled micromechanical system (MEMS) scanning mirror for laser scanning and aircore photonic crystal fiber for the delivery of femtosecond laser pulses. The system employs a miniaturized relay lens pair to limit aberrations due to pre-objective scanning and a separate, large numerical aperture fiber to enhance collection efficiency. The maximum FOV, lateral and axial resolution were measured to be 310 [Greek small letter mu]m and 1.64 [Greek small letter mu]m and 16.4 [Greek small letter mu]m, respectively. The two-photon imaging capabilities of this miniaturized probe were demonstrated using fluorescent beads and pollen grains.en_US
dc.description.departmentMechanical Engineeringen_US
dc.format.mediumelectronicen_US
dc.identifierdoi:10.15781/T2BZ61D6S
dc.identifier.urihttp://hdl.handle.net/2152/46098
dc.language.isoengen_US
dc.relation.ispartofUT Electronic Theses and Dissertationsen_US
dc.rightsCopyright © is held by the author. Presentation of this material on the Libraries' web site by University Libraries, The University of Texas at Austin was made possible under a limited license grant from the author who has retained all copyrights in the works.en_US
dc.rights.restrictionRestricteden_US
dc.subjectMiniaturized two-photon fluorescence microscope systemen_US
dc.subjectFemtosecond laser microsurgeryen_US
dc.subjectIn vivo diagnostic imagingen_US
dc.titleA miniaturized probe for combined two-photon microscopy and femtosecond laser microsurgeryen_US
dc.typeThesisen_US
dc.type.genreThesisen_US
thesis.degree.departmentMechanical Engineeringen_US
thesis.degree.disciplineMechanical Engineeringen_US
thesis.degree.grantorUniversity of Texas at Austinen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Scienceen_US

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