Microbial oxidation of arsenic : the potential for exploitation in drinking water treatment

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dc.contributor.advisorKirsits, Mary Jo
dc.contributor.advisorEaton, David J.
dc.creatorGreen, Cynthia Natalie, 1977-
dc.date.accessioned2017-02-21T20:21:02Z
dc.date.available2017-02-21T20:21:02Z
dc.date.issued2007-12
dc.description.abstractArsenic (As) contamination of drinking water is a problem prevalent throughout the world, and it has become more pertinent in the US following a recent reduction in the maximum contaminant limit (MCL) to 10 μg/L. Because As(V) is typically easier to remove than is As(III), arsenic is often chemically oxidized using free chlorine or permanganate. However, exploiting microorganisms to oxidize arsenic might have potential. In this study, microorganisms were isolated from water and filter media collected from the full-scale Greene County, Ohio drinking water treatment plant and from bench-scale filters seeded with filter media from the Greene County plant. These isolates were screened for arsenic-oxidation capability and resistance to arsenic. Molecular techniques, including 16S ribosomal RNA gene sequencing and terminal restriction fragment length polymorphism (T-RFLP) analysis, were used to phylogenetically identify and fingerprint the isolates, respectively. In addition, a gene probe targeting a region of the arsenite oxidase enzyme was used to demonstrate the presence of arsenic-oxidizing bacteria in the full-scale and bench-scale samples. Microbial arsenic-oxidizers could be an attractive alternative to the chemical oxidants that are traditionally used in arsenic treatment.en_US
dc.description.departmentCivil, Architectural, and Environmental Engineeringen_US
dc.description.departmentPublic Affairs
dc.format.mediumelectronicen_US
dc.identifierdoi:10.15781/T2KK94H6W
dc.identifier.urihttp://hdl.handle.net/2152/45734
dc.language.isoengen_US
dc.relation.ispartofUT Electronic Theses and Dissertationsen_US
dc.rightsCopyright © is held by the author. Presentation of this material on the Libraries' web site by University Libraries, The University of Texas at Austin was made possible under a limited license grant from the author who has retained all copyrights in the works.en_US
dc.rights.restrictionRestricteden_US
dc.subjectArsenic contamination of drinking wateren_US
dc.subjectMicrobial arsenic oxidizersen_US
dc.subjectArsenic treatment of drinking wateren_US
dc.titleMicrobial oxidation of arsenic : the potential for exploitation in drinking water treatmenten_US
dc.typeThesisen_US
dc.type.genreThesisen_US
thesis.degree.departmentCivil, Architectural, and Environmental Engineeringen_US
thesis.degree.departmentPublic Affairs
thesis.degree.disciplineCivil Engineeringen_US
thesis.degree.disciplinePublic Affairs
thesis.degree.grantorUniversity of Texas at Austinen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.nameMaster of Public Affairs

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