Precision Extruding Deposition and Characterization of Cellular Poly-e-Caprolactone Tissue Scaffolds

Successes in scaffold guided tissue engineering require scaffolds to have specific macroscopic geometries and internal architectures in order to provide the needed biological and biophysical functions. Freeform fabrication provides an effective process tool to manufacture many advanced scaffolds with designed properties. This paper reports our recent study on using a novel Precision Extruding Deposition (PED) process technique to directly fabricate cellular Poly-ε-Caprolactone (PCL) scaffolds. Scaffolds with a controlled pore size of 250 µm and designed structural orientations were fabricated. The scaffold morphology, internal micro-architecture and mechanical properties were evaluated using SEM, Micro-Computed Tomography (µ-CT) and the mechanical testing. Preliminary biological study was also conducted to investigate the cell responses to the as-fabricated tissue scaffolds. The results and the characterizations demonstrate the viability of the PED process to the scaffold fabrication as well as a good mechanical property, structural integrity, controlled pore size, pore interconnectivity, and the anticipated biological compatibility of the as-fabricated PCL scaffolds.