Extraction of Algal LIpids and their Analysis by HPLC and Mass Spectrometry

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dc.creatorJones, J.en
dc.creatorManning, S.en
dc.creatorMontoya, M.en
dc.creatorKeller, K.en
dc.creatorPoenie, M.en
dc.date.accessioned2015-09-04T16:06:10Zen
dc.date.available2015-09-04T16:06:10Zen
dc.date.issued2012-08en
dc.description.abstractAlgae are a promising source of biofuel but claims about their lipid content can be ambiguous because extraction methods vary and lipid quantitation often does not distinguish between particular lipid classes. Here we compared methods for the extraction of algal lipids and showed that 2-ethoxyethanol (2-EE) provides superior lipid recovery (>150–200 %) compared to other common extraction solvents such as chloroform:methanol or hexane. Extractions of wet and dry algal biomass showed that 2-EE was more effective at extracting lipids from wet rather than dried algal pellets. To analyze lipid content we used normal-phase HPLC with parallel quantitation by an evaporative light scattering detector and a mass spectrometer. Analysis of crude lipid extracts showed that all major lipid classes could be identified and quantified and revealed a surprisingly large amount of saturated hydrocarbons (HC). This HC fraction was isolated from extracts of bioreactor-grown algae and further analyzed by HPLC/MS, NMR, and GC/MS. The results showed that the sample consisted of a mixture of saturated, straight- and branched-chain HC of different chain lengths. These algal HC could represent an alternative biofuel to triacylglycerols (TAG) that could feed directly into the current petroleum infrastructure.en
dc.description.departmentCenter for Electromechanicsen
dc.identifierPR_553en
dc.identifier.citationJ. Jones, S. Manning, M. Montoya, K. Keller, and M. Poenie, “Extraction of Algal LIpids and their Analysis by HPLC and Mass Spectrometry,” Journal of the American Chemists Society, 2012, 89, 1371 - 1381, March 2012.en
dc.identifier.urihttp://hdl.handle.net/2152/30784en
dc.language.isoenen
dc.publisherSpringer Verlagen
dc.relation.ispartofCEM Publicationsen
dc.rights.restrictionopenen
dc.subjectBioproducts and Bioenergyen
dc.subjectAlgaeen
dc.subjectBiodieselen
dc.subjectBiofuelsen
dc.subjectLipidsen
dc.subjectELSDen
dc.subjectHPLC/MSen
dc.subjectGC/MSen
dc.subjectLipid extractionen
dc.subjectLipidomicsen
dc.titleExtraction of Algal LIpids and their Analysis by HPLC and Mass Spectrometryen
dc.typearticleen

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