Combined fluorescence and reflectance spectroscopy
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Date
2003-05-27
Authors
Rebecca R. Richards-Kortum
Anthony J. Durkin
Urs Utzinger
Holger Fuchs
Ann M. Gillenwater
Douglas L. Heintzelman
Journal Title
Journal ISSN
Volume Title
Publisher
United States Patent and Trademark Office
Abstract
Methods and apparatus for performing fluorescence and spatially resolved reflectance spectroscopy on a sample. A sample is irradiated with a fluorescence excitation fiber and radiation is collected from the sample with a fluorescence collection fiber and detected to form fluorescence spectra. The sample is also illuminated with a reflectance illumination fiber and reflected light from the sample is collected at a plurality of collection positions and detected to form spatially resolved reflectance spectra. The fibers may form a probe arranged in concentric sections. The spectra are analyzed by pre-processing and reducing the dimensionality of the spectral data.