Proteomic profiling of metalloenzymes via robust coordination of active-site metal
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Date
2018-01-29
Authors
Ngo, Chinh Quoc
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Abstract
Proteomic profiling of metalloenzymes in their native state is possible without using a covalent crosslinking group, if the interaction between the metal-binding group of the probe and the active-site metal is robust. In this proof-of-concept study, we show the feasibility of such approach with carbonic anhydrase as an example.