Browsing by Subject "amplification"
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Item A Dicationic Calix 4 Pyrrole Derivative and Its Use for the Selective Recognition and Displacement-Based Sensing of Pyrophosphate(2012-03) Sokkalingam, Punida; Kim, Dong Sub; Hwang, Hyonseok; Sessler, Jonathan L.; Lee, Cang-Hee; Kim, Dong Sub; Sessler, Jonathan L.A new bis-pyridinium calix[4] pyrrole derivative is reported. This system forms a non-fluorescent complex upon exposure to the chromenolate anion. The resulting supramolecular ensemble binds the pyrophosphate anion with high affinity (K-a (2.55 +/- 0.12) x 10(7) M-1) in acetonitrile. It exhibits sensitive "turn-on" fluorescence when exposed to tetrabutylammonium pyrophosphate, and does so in preference to other anions, including the fluoride and phosphate anions.Item Parametric Amplification Of Laser-Driven Acceleration In A Plasma Channel(2012-06) Arefiev, A. V.; Schollmeier, M.; Khudik, V. N.; Arefiev, Alexey V.; Khudik, Vladimir N.Two-dimensional particle-in-cell simulations are presented for a laser-irradiated solid-density target with and without an underdense preplasma. It is shown that an underdense preplasma can generate an energetic electron tail in addition to the warm electrons generated at the critical surface. Preplasma electrons are accelerated in a quasi-static positively charged channel formed by the laser. At ultra-relativistic laser intensities (a(0) = 10), the acceleration mechanism is not sensitive to the laser polarization. An energetic tail with energies significantly exceeding the energy expected for a single electron in a vacuum is present in simulations with s and p-polarized beams. This suggests that the mechanism of parametric amplification of laser-driven electron acceleration is a likely explanation for the observed phenomenon.Item Quantitative comparison of isothermal DNA amplification methods(2013) Wu, Yuefeng (Rose); Ellington, AndrewIn the field of isothermal DNA amplification, four of the widest used methods are recombinase polymerase amplification (RPA), helicase-dependent amplification (HDA), isothermal and chimeric primer initiated amplification of nucleic acids (ICAN) and loop-mediated isothermal amplification (LAMP). The areas of comparison between the four methods include robustness, limit of detection, specificity, ease of primer design, and reaction time. LAMP, developed in 2000 by Tsugunori Notomi et. al, has many characteristics that render it extremely attractive for therapeutic applications, clinical diagnosis, and easy DNA amplification. The presence of target DNA can be easily detected by a visual turbidity test, which can be a crucial advantage in a clinical diagnosis setting. Various primer sets, designed with the Primer Explore program (version 4), that target known genes of malaria and tuberculosis are tested in this project. Preliminary and quantitative real time experiments with primer sets for Microbacterium tuberculosis RPOB, Plasmodium falciparum CytB, and falciparum, ovale, malariae, and vivax 18S, using the standard LAMP protocol have yielded positive results. Similar analyses and experiments on the other three isothermal methods will reveal pros and cons of each technique, assisting others in making an educated decision on which method best suits their needs.