Browsing by Subject "algae-bacteria"

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Determining an effective method to select and observe an algae-bacteria symbiosis to bioremediate fluoranthene
(2023-04) Asmann, Noel Leah; Shear, Ruth
Bioremediation is the use of microorganisms to break down anthropogenic chemicals and is a sustainable and more rapid alternative to chemical decomposition. Fluoranthene is one of many polycyclic aromatic hydrocarbons (PAHs) created by pyrogenic mechanisms such as incomplete coal combustion, and is found in high abundance in urban waterways, like streams and creeks, through runoff. Several methods were investigated to form an efficient environment in which to develop a symbiotic system between Chlorella sorokiniana algae and Pseudomonas fluorescens bacteria that have been shown to be capable of bioremediating fluoranthene individually. Initial focus was placed on using solid media, in preparation to model bioremediation of PAHs using a microbial evolution and growth arena (MEGA) plate to encourage and track evolution of the symbiotic system over time. Analysis of solid media showed fluoranthene distribution was non-uniform; however, the use of liquid media appears to be a viable alternative. Bioremediation in liquid cultures was observed during exposure to fluoranthene suggesting that this may be a viable tool for further development of these organisms for bioremediation. The ever-increasing presence of anthropogenic pollutants in urban waterway ecosystems, requires our urgent attention and environmentally friendly remediation methods are a highly desirable and promising solution.