Browsing by Subject "Zinc-finger proteins"
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Item Functional studies of BCL11A: a transcriptional repressor implicated in chromosome 2p13-disrupted malignancy(2002) Liu, Hui; Tucker, Philip W.Many malignancies of mature B lymphocytes are characterized by chromosomal translocations involving the immunoglobulin heavy chain (IGH) locus on chromosome 14q32.2, resulting in the deregulation of the translocated proto-oncogene. Although t(2;14)(p13;q32.3) is a rare event in B cell malignancies, gains and amplifications of chromosome 2p13 have been reported in 20% of extranodal B cell non-Hodgkin’s lymphoma (B-NHL) and in Hodgkin’s disease (HD). In previous studies, we have identified a novel Krüppel zinc finger gene BCL11A and showed it to be disrupted and deregulated in four cases of B cell malignancies with t(2;14)(p13;q32.3). Three major alternatively spliced isoforms of BCL11A have been detected: BCL11Axl (~120 kD), BCL11Al (~100 kD) and BCL11As (~30 kD). All three mRNAs were upregulated as a consequence of the t(2;14)(p13;q32). Here we confirm that at the protein level, BCL11A isoforms are overexpressed in malignant cells in which the 2p13 locus is genetically disrupted. We also demonstrate that within normal immune tissues, BCL11A accumulates preferentially in germinal centers. In addition, all three isoforms can physically interact with each other. Both BCL11Axl and BCL11Al can colocalize and physically interact with another human B cell proto-oncogene BCL6. BCL11A isoforms potentiate transcriptional repression activity, which is trichostatin A and BCL6 independent. BCL11Axl and BCL11Al but not BCL11As can induce apoptosis in both lymphoid and nonlymphoid cell lines. The apoptotic effect is independent of BCL6, but can be moderately enhanced by p53, and completely blocked by the overexpression of Bcl-2 or a caspase-9 inhibitor. Potential target genes for BCL11Axl were identified by screening Lymphochip microarrays. 28 out of 39 target genes were down-regulated by BCL11Axl. A number of genes functioning in signal transduction, cell cycle control and apoptosis were identified, including Akt2, ATR, Trio, NFκB, and MDM2. Based on the data cited above, we propose that BCL11A induces apoptosis through blockage of the PI3K-Akt cell survival pathway. Although the mechanism by which deregulated BCL11A causes B cell malignancies remains elusive, we suggest that the inhibition of cell cycle checkpoint pathways and Rac-Rho signaling pathways via down regulation of ATR and Trio might play critical roles in BCL11A tumorigenesis.Item Zinc-finger transcription factors and the response of non-myelinating Schwann cells to axonal injury(2008-05) Ellerton, Elaine Louise; Rimer, Mendell; Thompson, Wesley J.Schwann cell (SCs) are the glia of the peripheral nervous system. During development, a common precursor develops into two distinct types of SCs: myelinating SCs and non-myelinating SCs. There is little literature regarding the non-myelinating variety of SCs, specifically a type of non-myelinating SC found at the neuromuscular junction (NMJ), the terminal Schwann cell (tSC). Terminal SCs are critical for the maintenance and recovery of the NMJ. Peripheral nerve injury causes tSCs to become reactive, a state characterized by changes in gene expression and the extension of cellular processes. It is in this state, that tSCs help to restore functionality to the denervated junction. What drives tSCs to become reactive after injury remains largely unknown. Previously, nine zinc-finger proteins (ZFPs), a class of transcription factors, have been implicated in SC development and differentiation. As a result, transcription factors from the ZFP family were considered as potential candidates that may drive the activation of tSCs. Because tSCs are few and far between, only two-six cells cover the NMJ, I used a largely non-myelinated nerve of the autonomic system, the cervical sympathetic trunk (CST), to search for ZFP candidates. I created a cDNA library from both control and denervated CST resulting in 40 unique ZFPs. Six of these genes were studied futher: Zipro1, Zfp36, Zfp612, Zfp180, Zfp111 and Zfp629. I found a near two-fold increase in Zipro1 mRNA and protein in denervated CST, and no change in the other five ZFPs (results from Zfp629 remain inconclusive). Using an antibody against Zipro1, I located this increase of Zipro1 to the SCs of the denervated CST. I also found Zipro1 expression in tSCs and an increase of 19% in tSCs of denervated rat muscle. Upregulation of Zipro1 in non-myelinating SCs suggests that Zipro1 may have a role in the activation of tSCs. Further study is needed in order to clarify the extent of Zipro1's involvement.