Browsing by Subject "Multiphoton polymerization"
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Item Toward the study of dendritic protein synthesis by manipulating subcellular mRNA localization(2004-08-16) Li, Jinying; Shear, Jason B.In neuronal cells, the dendritic local translation of proteins may play a role in memory formation by contributing to certain types of synaptic plasticity. We seek to probe dendritic local protein synthesis inside neuronal cells, by taking advantages of a novel laser-tweezer based subcellular perturbation and analysis system. In this system, a microparticle decorated with signal mRNA is introduced into a single neuronal cell, producing detectable cellular responses. In this thesis, it is demonstrated that successful particle introduction using laser tweezers has been achieved, that the chemistry of immobilizing biologically active mRNA molecules onto microsphere surface was optimized, and that the particle-immobilized mRNA retained translation activity inside neuronal cells. The microfabrication technique based on multiphoton excited protein crosslinking provides a possibility of improving the cell viability for the laser-tweezers system by creating more biocompatible materials. To prove that, avidin micro-structures were created by multiphoton polymerization and were demonstrated to efficiently capture biotinylated mRNA