Insights into the evolution of mammalian telomerase: Platypus TERT shares similarities with genes of birds and other reptiles and localizes on sex chromosomes

dc.creatorHrdlickova, Radmilaen
dc.creatorNehyba, Jirien
dc.creatorLim, Shu Lyen
dc.creatorGrutzner, Franken
dc.creatorBose, Henry R.en
dc.date.accessioned2014-12-15T17:09:40Zen
dc.date.available2014-12-15T17:09:40Zen
dc.date.issued2012-06-01en
dc.descriptionRadmila Hrdličková, Jiří Nehyba and Henry R Bose are with the Section of Molecular Genetics and Microbiology, School of Biological Science, and Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712-1095, USA -- Shu Ly Lim and Frank Grützner The Robinson Institute, School of Molecular and Biomedical Science, The University of Adelaide, Adelaide, 5005 SA, Australiaen
dc.description.abstractBackground: The TERT gene encodes the catalytic subunit of the telomerase complex and is responsible for maintaining telomere length. Vertebrate telomerase has been studied in eutherian mammals, fish, and the chicken, but less attention has been paid to other vertebrates. The platypus occupies an important evolutionary position, providing unique insight into the evolution of mammalian genes. We report the cloning of a platypus TERT (OanTERT) ortholog, and provide a comparison with genes of other vertebrates. Results: The OanTERT encodes a protein with a high sequence similarity to marsupial TERT and avian TERT. Like the TERT of sauropsids and marsupials, as well as that of sharks and echinoderms, OanTERT contains extended variable linkers in the N-terminal region suggesting that they were present already in basal vertebrates and lost independently in ray-finned fish and eutherian mammals. Several alternatively spliced OanTERT variants structurally similar to avian TERT variants were identified. Telomerase activity is expressed in all platypus tissues like that of cold-blooded animals and murine rodents. OanTERT was localized on pseudoautosomal regions of sex chromosomes X3/Y2, expanding the homology between human chromosome 5 and platypus sex chromosomes. Synteny analysis suggests that TERT co-localized with sex-linked genes in the last common mammalian ancestor. Interestingly, female platypuses express higher levels of telomerase in heart and liver tissues than do males. Conclusions: OanTERT shares many features with TERT of the reptilian outgroup, suggesting that OanTERT represents the ancestral mammalian TERT. Features specific to TERT of eutherian mammals have, therefore, evolved more recently after the divergence of monotremes.en
dc.description.catalogingnotebose@mail.utexas.eduen
dc.description.departmentCellular and Molecular Biologyen
dc.description.sponsorshipen
dc.identifier.Filename1471-2164-13-216.pdfen
dc.identifier.citationHrdličková, Radmila, Jiří Nehyba, Shu Ly Lim, Frank Grützner, and Henry R. Bose. “Insights into the Evolution of Mammalian Telomerase: Platypus TERT Shares Similarities with Genes of Birds and Other Reptiles and Localizes on Sex Chromosomes.” BMC Genomics 13, no. 1 (June 1, 2012): 216. doi:10.1186/1471-2164-13-216.en
dc.identifier.doidoi:10.1186/1471-2164-13-216en
dc.identifier.urihttp://hdl.handle.net/2152/27784en
dc.language.isoEnglishen
dc.publisherBMC Genomicsen
dc.rightsAdministrative deposit of works to UT Digital Repository: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access at http://www.biomedcentral.com. The public license is specified as CC-BY: http://creativecommons.org/licenses/by/4.0/. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en
dc.subjectPlatypusen
dc.subjectTERTen
dc.subjectTelomeraseen
dc.subjectAlternative splicingen
dc.subjectTelomeresen
dc.subjectSex chromosomesen
dc.titleInsights into the evolution of mammalian telomerase: Platypus TERT shares similarities with genes of birds and other reptiles and localizes on sex chromosomesen
dc.typeArticleen

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