Pyrosequencing To Detect Mutations In FKS1 That Confer Reduced Echinocandin Susceptibility In Candida Albicans

Date

2008-11

Authors

Wiederhold, Nathan P.
Grabinski, Jodi L.
Garcia-Effron, Guillermo
Perlin, David S.
Lee, Samuel A.

Journal Title

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Volume Title

Publisher

Abstract

Pyrosequencing was compared to Sanger dideoxy sequencing to detect mutations in FKS1 responsible for reduced echinocandin susceptibility in Candida albicans. These methods were in complete agreement for 10 of 12 clinical isolates with elevated echinocandin MICs, supporting the potential feasibility of pyrosequencing to detect mutations within diploid fungi.

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Citation

Wiederhold, N. P., Grabinski, J. L., Garcia-Effron, G., Perlin, D. S., & Lee, S. A. Pyrosequencing To Detect Mutations In FKS1 That Confer Reduced Echinocandin Susceptibility In Candida Albicans. Antimicrobial Agents and Chemotherapy, Vol. 52, No. 11 (Nov., 2008) pp. 4145–4148. doi:10.1128/AAC.00959-08