Dielectrophoresis has Broad Applicability to Marker-Free Isolation of Tumor Cells from Blood by Microfluidic Systems

dc.contributor.utaustinauthorShim, Sangjoen_US
dc.creatorShim, Sangjoen_US
dc.creatorStemke-Hale, Katherineen_US
dc.creatorNoshari, Jamilehen_US
dc.creatorBecker, Frederick F.en_US
dc.creatorGascoyne, Peter R. C.en_US
dc.date.accessioned2016-10-28T19:50:34Z
dc.date.available2016-10-28T19:50:34Z
dc.date.issued2013-01en_US
dc.description.abstractThe number of circulating tumor cells (CTCs) found in blood is known to be a prognostic marker for recurrence of primary tumors, however, most current methods for isolating CTCs rely on cell surface markers that are not universally expressed by CTCs. Dielectrophoresis (DEP) can discriminate and manipulate cancer cells in microfluidic systems and has been proposed as a molecular marker-independent approach for isolating CTCs from blood. To investigate the potential applicability of DEP to different cancer types, the dielectric and density properties of the NCI-60 panel of tumor cell types have been measured by dielectrophoretic field-flow fractionation (DEP-FFF) and compared with like properties of the subpopulations of normal peripheral blood cells. We show that all of the NCI-60 cell types, regardless of tissue of origin, exhibit dielectric properties that facilitate their isolation from blood by DEP. Cell types derived from solid tumors that grew in adherent cultures exhibited dielectric properties that were strikingly different from those of peripheral blood cell subpopulations while leukemia-derived lines that grew in non-adherent cultures exhibited dielectric properties that were closer to those of peripheral blood cell types. Our results suggest that DEP methods have wide applicability for the surface-marker independent isolation of viable CTCs from blood as well as for the concentration of leukemia cells from blood. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4774307]en_US
dc.description.departmentBiomedical Engineeringen_US
dc.description.sponsorshipCancer Prevention and Research Institute of Texas (CPRIT) RP100934en_US
dc.description.sponsorshipKleberg Center for Molecular Markersen_US
dc.description.sponsorshipEntertainment Industry Foundation SU2C-AACR-DT0209en_US
dc.description.sponsorshipNCI CA016672en_US
dc.identifierdoi:10.15781/T2SJ19T7Z
dc.identifier.citationShim, Sangjo, Katherine Stemke-Hale, Jamileh Noshari, Frederick F. Becker, and Peter RC Gascoyne. "Dielectrophoresis has broad applicability to marker-free isolation of tumor cells from blood by microfluidic systems." Biomicrofluidics, Vol. 7, No. 1 (Jan., 2013): 011808.en_US
dc.identifier.doi10.1063/1.4774307en_US
dc.identifier.issn1932-1058en_US
dc.identifier.urihttp://hdl.handle.net/2152/43201
dc.language.isoEnglishen_US
dc.relation.ispartofen_US
dc.relation.ispartofserialBiomicrofluidicsen_US
dc.rightsAdministrative deposit of works to Texas ScholarWorks: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access or the publisher allows a PDF version of the article to be freely posted online. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en_US
dc.rights.restrictionOpenen_US
dc.subjectbioelectric phenomenaen_US
dc.subjectbiological techniquesen_US
dc.subjectbiomemsen_US
dc.subjectcanceren_US
dc.subjectcellularen_US
dc.subjectbiophysicsen_US
dc.subjectelectrophoresisen_US
dc.subjecthaemodynamicsen_US
dc.subjectmicrofluidicsen_US
dc.subjectmicroorganismsen_US
dc.subjecttumoursen_US
dc.subjectfield-flow-fractionationen_US
dc.subjectbreast-cancer cellsen_US
dc.subjectprostate-canceren_US
dc.subjectshear-flowen_US
dc.subjectstem-cellsen_US
dc.subjectseparationen_US
dc.subjectelectrorotationen_US
dc.subjectpanelen_US
dc.subjectexpressionen_US
dc.subjectcarcinomaen_US
dc.subjectbiochemical research methodsen_US
dc.subjectbiophysicsen_US
dc.subjectnanoscience & nanotechnologyen_US
dc.subjectphysics, fluids & plasmasen_US
dc.titleDielectrophoresis has Broad Applicability to Marker-Free Isolation of Tumor Cells from Blood by Microfluidic Systemsen_US
dc.typeArticleen_US

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