Membrane-Potential of Rat Hepatoma-Cells in Culture - Influence of Factors Affecting Amino-Acid-Transport

dc.contributor.utaustinauthorMouat, M. F.en_US
dc.creatorMouat, M. F.en_US
dc.creatorCantrell, A. C.en_US
dc.creatorManchester, K. L.en_US
dc.date.accessioned2016-10-28T19:52:43Z
dc.date.available2016-10-28T19:52:43Z
dc.date.issued1995-08en_US
dc.description.abstractThe effect has been studied of various media, hormones and of amino acids on the membrane potential of rat hepatoma cells in culture measured by microelectrode impalement. Cells in Eagle's minimal essential medium plus 5% serum had a value which varied daily from about 5-8 mV, inside negative. The membrane potential of rat hepatocytes was measured to be 8.7+/-0.2mV, inside negative. The membrane potential of the hepatoma cells was decreased by insulin and increased by glucagon. Membrane potential was unaffected by change of medium to Hanks' or Earle's balanced salt solutions or deprivation of serum. It was, however, reduced in cells in phosphate-buffered saline and by reduction of pH. The former effect was shown to be due to the higher [Na+] of phosphate-buffered saline as opposed to the other media. Addition of alanine, glycine, serine, proline and methylaminoisobutyrate all reduced membrane potential by 2-3 mV. Smaller decreases were seen with methionine, leucine and phenylalanine, but none with glutamine, threonine, BCH (2-aminonorborane-2-carboxylic acid) and D-alanine. The results are compared with the effects of similar conditions on aminoisobutyrate uptake. Whilst there was a correlation under some conditions there was not under others. It is concluded that for the hepatoma cells factors additional to the membrane potential must exert some influence on the capacity for amino acid transport.en_US
dc.description.departmentHuman Ecologyen_US
dc.description.sponsorshipSouth African Council for Scientific and Industrial Researchen_US
dc.identifierdoi:10.15781/T23F4KQ9S
dc.identifier.citationMouat, M. F., A. C. Cantrell, and K. L. Manchester. "Membrane potential of rat hepatoma cells in culture: Influence of factors affecting amino acid transport." Bioscience reports, Vol. 15, No. 4 (Aug., 1995): 173-184.en_US
dc.identifier.doi10.1007/bf01540451en_US
dc.identifier.issn0144-8463en_US
dc.identifier.urihttp://hdl.handle.net/2152/43307
dc.language.isoEnglishen_US
dc.relation.ispartofen_US
dc.relation.ispartofserialBioscience Reportsen_US
dc.rightsAdministrative deposit of works to Texas ScholarWorks: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access or the publisher allows a PDF version of the article to be freely posted online. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en_US
dc.rights.restrictionOpenen_US
dc.subjectmicroelectrodeen_US
dc.subjectsodium ionen_US
dc.subjectaminoisobutyrate uptakeen_US
dc.subjectascites-tumor-cellsen_US
dc.subjectplasma-membraneen_US
dc.subjectmouse fibroblastsen_US
dc.subjecthepatocytesen_US
dc.subjectsystemen_US
dc.subjectdepolarizationen_US
dc.subjectvesiclesen_US
dc.subjectinsulinen_US
dc.subjectliveren_US
dc.subjectcell biologyen_US
dc.titleMembrane-Potential of Rat Hepatoma-Cells in Culture - Influence of Factors Affecting Amino-Acid-Transporten_US
dc.typeArticleen_US

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