Use of template switching for DNA synthesis

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dc.contributor.assigneeBoard of Regents, The University of Texas System
dc.creatorAlan M. Lambowitz
dc.creatorScott Kuersten
dc.creatorSabine Mohr
dc.creatorTravis B. White
dc.date.accessioned2019-10-23T14:33:14Z
dc.date.available2019-10-23T14:33:14Z
dc.date.filed2012-02-23
dc.date.issued2015-04-21
dc.description.abstractA method of preparing a DNA copy of a target polynucleotide using template switching is described. The method includes mixing a double stranded template/primer substrate made up of a DNA primer oligonucleotide associated with a complementary oligonucleotide template strand with a target polynucleotide in a reaction medium and adding a suitable amount of a non-retroviral reverse transcriptase to the reaction medium to extend the DNA primer oligonucleotide from its 3′ end to provide a DNA copy polynucleotide. The DNA copy polynucleotide includes a complementary target DNA polynucleotide that is synthesized using the target polynucleotide as a template. Methods of adding nucleotides to the double stranded template/primer substrate are also described. The method can be used to facilitate detection, PCR amplification, cloning, and determination of RNA and DNA sequences.
dc.description.departmentBoard of Regents, University of Texas System
dc.identifier.applicationnumber14000513
dc.identifier.patentnumber9012183
dc.identifier.urihttps://hdl.handle.net/2152/76661
dc.identifier.urihttp://dx.doi.org/10.26153/tsw/3750
dc.publisherUnited States Patent and Trademark Office
dc.relation.ispartofUniversity of Texas Patents
dc.relation.ispartofUniversity of Texas Patents
dc.rights.restrictionOpen
dc.rights.restrictionOpen
dc.subject.cpcC12P19/34
dc.subject.cpcC12N15/1096
dc.subject.cpcC12Q1/6806
dc.subject.cpcC12Q2521/107
dc.subject.cpcC12Q2525/191
dc.subject.cpcC12Q2525/207
dc.subject.uspc435/91.1
dc.subject.uspc435/91.2
dc.titleUse of template switching for DNA synthesis
dc.typePatent

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