Identification of genes that interact with liquid facets

dc.contributor.advisorFischer, Janice Ann
dc.creatorVan Der Ende, Gerrit Alexanderen
dc.date.accessioned2014-02-03T17:07:24Zen
dc.date.issued2012-08en
dc.date.submittedAugust 2012en
dc.date.updated2014-02-03T17:07:25Zen
dc.descriptiontexten
dc.description.abstractThe protein Liquid facets (Lqf) promotes endocytosis at the plasma membrane1. Lqf activity is required for proper Notch signaling, likely through facilitating the endocytosis of Notch ligand by indirectly linking ligand to clathrin. A genetic modifier screen to identify genes that interact with lqf was performed by a previous graduate student. Genes identified in the screen might provide new insights into how Lqf promotes endocytosis or how Notch signaling is regulated. In this work, I performed genetic mapping techniques to identify the genes mutated in each complementation group of the screen. I identified the gene mutated in complementation group 6 as mitochondrial alanyl tRNA synthetase (Aats-ala-m). tRNA synthetases link a tRNA to its cognate amino acid during translation. Mitochondrial tRNA synthetases function in the mitochondria in translation. Aats-ala-m genetically interacts with lqf, suggesting the two genes function in the same pathway. In this work, I also identified chromosomal regions where the genes mutated in complementation groups 1,2, and 9 are located.en
dc.description.departmentCellular and Molecular Biologyen
dc.format.mimetypeapplication/pdfen
dc.identifier.urihttp://hdl.handle.net/2152/23020en
dc.language.isoen_USen
dc.subjectLiquid facetsen
dc.subjectEpsinen
dc.subjectMappingen
dc.subjectNotch signalingen
dc.subjectMitochondrial aminoacyl tRNA synthetaseen
dc.titleIdentification of genes that interact with liquid facetsen
thesis.degree.departmentCellular and Molecular Biologyen
thesis.degree.disciplineCell and Molecular Biologyen
thesis.degree.grantorThe University of Texas at Austinen
thesis.degree.levelMastersen
thesis.degree.nameMaster of Artsen

Access full-text files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
thesis1.pdf
Size:
1.06 MB
Format:
Adobe Portable Document Format

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.13 KB
Format:
Plain Text
Description: