Routes Of Acquisition Of The Gut Microbiota Of The Honey Bee Apis Mellifera

Simple item page
dc.contributor.utaustinauthorPowell, J. Elijahen
dc.contributor.utaustinauthorMoran, Nancy A.en
dc.creatorPowell, J. Elijahen
dc.creatorMartinson, Vincent G.en
dc.creatorUrban-Mead, Katherineen
dc.creatorMoran, Nancy A.en
dc.date.accessioned2015-09-09T15:50:06Zen
dc.date.available2015-09-09T15:50:06Zen
dc.date.issued2014-12en
dc.description.abstractStudies of newly emerged Apis mellifera worker bees have demonstrated that their guts are colonized by a consistent core microbiota within several days of eclosure. We conducted experiments aimed at illuminating the transmission routes and spatiotemporal colonization dynamics of this microbiota. Experimental groups of newly emerged workers were maintained in cup cages and exposed to different potential transmission sources. Colonization patterns were evaluated using quantitative real-time PCR (qPCR) to assess community sizes and using deep sequencing of 16S rRNA gene amplicons to assess community composition. In addition, we monitored the establishment of the ileum and rectum communities within workers sampled over time from natural hive conditions. The study verified that workers initially lack gut bacteria and gain large characteristic communities in the ileum and rectum within 4 to 6 days within hives. Typical communities, resembling those of workers within hives, were established in the presence of nurse workers or nurse worker fecal material, and atypical communities of noncore or highly skewed compositions were established when workers were exposed only to oral trophallaxis or hive components (comb, honey, bee bread). The core species of Gram-negative bacteria, Snodgrassella alvi, Gilliamella apicola, and Frischella perrara, were dependent on the presence of nurses or hindgut material, whereas some Gram-positive species were more often transferred through exposure to hive components. These results indicate aspects of the colony life cycle and behavior that are key to the propagation of the characteristic honey bee gut microbiota.en
dc.description.departmentIntegrative Biologyen
dc.description.sponsorshipNational Science Foundation 1046153, 1415604en
dc.description.sponsorshipUniversity of Texas at Austinen
dc.identifier.citationPowell, J. Elijah, Martinson, Vincent G., Urban-Mead, Katherine, Moran, Nancy A., >Routes of Acquisition of the Gut Microbiota of the Honey Bee Apis mellifera,> Appl. Environ. Microbiol. December 2014 vol. 80 no. 23 7378-7387. doi: 10.1128/AEM.01861-14.en
dc.identifier.doi10.1128/aem.01861-14en
dc.identifier.issn0099-2240en
dc.identifier.urihttp://hdl.handle.net/2152/31054en
dc.identifier.urlen
dc.language.isoEnglishen
dc.relation.ispartofserialApplied and Environmental Microbiologyen
dc.rightsAdministrative deposit of works to Texas ScholarWorks: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access or the publisher allows a PDF version of the article to be freely posted online. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en
dc.rights.holderen
dc.subjectdisease resistanceen
dc.subjectsocial immunityen
dc.subjectbumble beesen
dc.subjectbacteriaen
dc.subjectdiversityen
dc.subjectlactobacillien
dc.subjecttransmissionen
dc.subjectprophylaxisen
dc.subjectcommunitiesen
dc.subjecthymenopteraen
dc.subjectbiotechnology & applied microbiologyen
dc.subjectmicrobiologyen
dc.titleRoutes Of Acquisition Of The Gut Microbiota Of The Honey Bee Apis Melliferaen
dc.typeArticleen

Access full-text files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
2014_12_Routes.pdf
Size:
682.48 KB
Format:
Adobe Portable Document Format
Download

Collections

UT Faculty/Researcher Works