Photosynthetic gene Lhcf2 mRNA stability control in response to a herbivory induced aldehyde in a marine diatom
| dc.contributor.advisor | Mehdy, Mona Cynthia, 1955- | |
| dc.contributor.committeeMember | Herrin, David | |
| dc.creator | Islam, Shahima | |
| dc.date.accessioned | 2022-09-13T19:28:06Z | |
| dc.date.available | 2022-09-13T19:28:06Z | |
| dc.date.created | 2015-08 | |
| dc.date.issued | 2015-09-11 | |
| dc.date.submitted | August 2015 | |
| dc.date.updated | 2022-09-13T19:28:08Z | |
| dc.description.abstract | Dynamic challenges of biotic stress agents are prevalent in the life of a photosynthetic organism. One widely observed biotic stress defense mechanism is down- regulation of photosynthetic gene expression, but the mechanisms of down- regulation are unknown. In this study, the mechanism of photosynthetic gene regulation of a light harvesting complex gene was studied in the marine diatom Phaeodactylum tricornatum in response to a diatom derived toxic aldehyde, 2E,4E-decadienal (DD), which is released in response to herbivory. We have shown that Lhcf2, a strongly expressed LHC gene in P. tricornutum, was down-regulated to around 5 % within 1hr of DD treatment and 10-15% of original mRNA levels after 6 hr. No change in Lhcf2 transcription rates were detectable between the control and DD treated cells. Instead, assays of post-transcriptional mRNA metabolism showed that DD treatment reduced the Lhcf2 mRNA half-life. To our knowledge, this is the first report on the mechanism of biotic stress-induced photosynthetic gene down-regulation | |
| dc.description.department | Plant Biology | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | https://hdl.handle.net/2152/115682 | |
| dc.identifier.uri | http://dx.doi.org/10.26153/tsw/42580 | |
| dc.language.iso | en | |
| dc.subject | Photosynthesis | |
| dc.subject | Biotic stress | |
| dc.subject | Light harvesting complex | |
| dc.subject | Transcription | |
| dc.subject | mRNA half-life | |
| dc.title | Photosynthetic gene Lhcf2 mRNA stability control in response to a herbivory induced aldehyde in a marine diatom | |
| dc.type | Thesis | |
| dc.type.material | text | |
| thesis.degree.department | Plant Biology | |
| thesis.degree.discipline | Plant Biology | |
| thesis.degree.grantor | The University of Texas at Austin | |
| thesis.degree.level | Masters | |
| thesis.degree.name | Master of Arts |