Photosynthetic gene Lhcf2 mRNA stability control in response to a herbivory induced aldehyde in a marine diatom

dc.contributor.advisorMehdy, Mona Cynthia, 1955-
dc.contributor.committeeMemberHerrin, David
dc.creatorIslam, Shahima
dc.date.accessioned2022-09-13T19:28:06Z
dc.date.available2022-09-13T19:28:06Z
dc.date.created2015-08
dc.date.issued2015-09-11
dc.date.submittedAugust 2015
dc.date.updated2022-09-13T19:28:08Z
dc.description.abstractDynamic challenges of biotic stress agents are prevalent in the life of a photosynthetic organism. One widely observed biotic stress defense mechanism is down- regulation of photosynthetic gene expression, but the mechanisms of down- regulation are unknown. In this study, the mechanism of photosynthetic gene regulation of a light harvesting complex gene was studied in the marine diatom Phaeodactylum tricornatum in response to a diatom derived toxic aldehyde, 2E,4E-decadienal (DD), which is released in response to herbivory. We have shown that Lhcf2, a strongly expressed LHC gene in P. tricornutum, was down-regulated to around 5 % within 1hr of DD treatment and 10-15% of original mRNA levels after 6 hr. No change in Lhcf2 transcription rates were detectable between the control and DD treated cells. Instead, assays of post-transcriptional mRNA metabolism showed that DD treatment reduced the Lhcf2 mRNA half-life. To our knowledge, this is the first report on the mechanism of biotic stress-induced photosynthetic gene down-regulation
dc.description.departmentPlant Biology
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/2152/115682
dc.identifier.urihttp://dx.doi.org/10.26153/tsw/42580
dc.language.isoen
dc.subjectPhotosynthesis
dc.subjectBiotic stress
dc.subjectLight harvesting complex
dc.subjectTranscription
dc.subjectmRNA half-life
dc.titlePhotosynthetic gene Lhcf2 mRNA stability control in response to a herbivory induced aldehyde in a marine diatom
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentPlant Biology
thesis.degree.disciplinePlant Biology
thesis.degree.grantorThe University of Texas at Austin
thesis.degree.levelMasters
thesis.degree.nameMaster of Arts
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