In vitro scanning saturation mutagenesis of proteins

dc.contributor.assigneeBoard of Regents, The University of Texas System
dc.creatorBrent L. Iverson
dc.creatorGeorge M. Georgiou
dc.creatorElizabeth A. Burks
dc.date.accessioned2019-10-23T21:40:38Z
dc.date.available2019-10-23T21:40:38Z
dc.date.filed1998-04-30
dc.date.issued2001-01-30
dc.description.abstractThe present invention combines PCR.TM. mutagenesis with in vitro transcription/translation and ELISA for the rapid generation and characterization of protein mutants. The PCR.TM. products are used directly as the template for the in vitro transcription/translation reactions and because no cloning steps are required, the in vitro saturation mutagenesis of one residue can be completed in duplicate within a week by a single investigator. This high throughput enables the saturation mutagenesis of numerous residues of interest, a process that can be described as in vitro scanning saturation mutagenesis. Compositions and methods of use of such a process are described herein.
dc.description.departmentBoard of Regents, University of Texas System
dc.identifier.applicationnumber9070408
dc.identifier.patentnumber6180341
dc.identifier.urihttps://hdl.handle.net/2152/77245
dc.identifier.urihttp://dx.doi.org/10.26153/tsw/4334
dc.publisherUnited States Patent and Trademark Office
dc.relation.ispartofUniversity of Texas Patents
dc.relation.ispartofUniversity of Texas Patents
dc.rights.restrictionOpen
dc.rights.restrictionOpen
dc.subject.cpcC07K16/00
dc.subject.cpcC07K16/16
dc.subject.cpcC07K2317/622
dc.subject.cpcC12N15/1093
dc.subject.cpcC12N15/102
dc.subject.uspc435/6.14
dc.subject.uspc711/100
dc.subject.uspc711/112
dc.subject.uspc711/114
dc.subject.uspc711/147
dc.titleIn vitro scanning saturation mutagenesis of proteins
dc.typePatent

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