Antibody-Independent Isolation of Circulating Tumor Cells by Continuous-Flow Dielectrophoresis

dc.contributor.utaustinauthorShim, Sangjoen_US
dc.contributor.utaustinauthorNoshari, Jamilehen_US
dc.contributor.utaustinauthorAnderson, Thomas E.en_US
dc.contributor.utaustinauthorGascoyne, Peter R. C.en_US
dc.creatorShim, Sangjoen_US
dc.creatorStemke-Hale, Katherineen_US
dc.creatorTsimberidou, Apostolia M.en_US
dc.creatorNoshari, Jamilehen_US
dc.creatorAnderson, Thomas E.en_US
dc.creatorGascoyne, Peter R. C.en_US
dc.date.accessioned2016-10-28T19:51:28Z
dc.date.available2016-10-28T19:51:28Z
dc.date.issued2013-01en_US
dc.description.abstractCirculating tumor cells (CTCs) are prognostic markers for the recurrence of cancer and may carry molecular information relevant to cancer diagnosis. Dielectrophoresis (DEP) has been proposed as a molecular marker-independent approach for isolating CTCs from blood and has been shown to be broadly applicable to different types of cancers. However, existing batch-mode microfluidic DEP methods have been unable to process 10 ml clinical blood specimens rapidly enough. To achieve the required processing rates of 106 nucleated cells/min, we describe a continuous flow microfluidic processing chamber into which the peripheral blood mononuclear cell fraction of a clinical specimen is slowly injected, deionized by diffusion, and then subjected to a balance of DEP, sedimentation and hydrodynamic lift forces. These forces cause tumor cells to be transported close to the floor of the chamber, while blood cells are carried about three cell diameters above them. The tumor cells are isolated by skimming them from the bottom of the chamber while the blood cells flow to waste. The principles, design, and modeling of the continuous-flow system are presented. To illustrate operation of the technology, we demonstrate the isolation of circulating colon tumor cells from clinical specimens and verify the tumor origin of these cells by molecular analysis. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4774304]en_US
dc.description.departmentImaging Research Centeren_US
dc.description.sponsorshipCancer Prevention and Research Institute of Texas (CPRIT) RP100934en_US
dc.description.sponsorshipKleberg Center for Molecular Markersen_US
dc.description.sponsorshipApoCell, Incen_US
dc.description.sponsorshipEntertainment Industry Foundation SU2C-AACR-DT0209en_US
dc.identifierdoi:10.15781/T23R0PW8C
dc.identifier.citationShim, Sangjo, Katherine Stemke-Hale, Apostolia M. Tsimberidou, Jamileh Noshari, Thomas E. Anderson, and Peter RC Gascoyne. "Antibody-independent isolation of circulating tumor cells by continuous-flow dielectrophoresis." Biomicrofluidics, Vol. 7, No. 1 (Jan., 2013): 011807.en_US
dc.identifier.doi10.1063/1.4774304en_US
dc.identifier.issn1932-1058en_US
dc.identifier.urihttp://hdl.handle.net/2152/43245
dc.language.isoEnglishen_US
dc.relation.ispartofen_US
dc.relation.ispartofserialBiomicrofluidicsen_US
dc.rightsAdministrative deposit of works to Texas ScholarWorks: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access or the publisher allows a PDF version of the article to be freely posted online. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en_US
dc.rights.restrictionOpenen_US
dc.subjectbioelectric phenomenaen_US
dc.subjectbiomemsen_US
dc.subjectblooden_US
dc.subjectcellular transporten_US
dc.subjectdiffusionen_US
dc.subjectelectrophoresisen_US
dc.subjecthydrodynamicsen_US
dc.subjectmicrofluidicsen_US
dc.subjectmolecular biophysicsen_US
dc.subjectpatient diagnosisen_US
dc.subjectsedimentationen_US
dc.subjecttumoursen_US
dc.subjectbreast-cancer cellsen_US
dc.subjectstem-cellsen_US
dc.subjectfielden_US
dc.subjectfractionationen_US
dc.subjectseparationen_US
dc.subjectblooden_US
dc.subjectelectrorotationen_US
dc.subjectremovalen_US
dc.subjectmodelen_US
dc.subjectbiochemical research methodsen_US
dc.subjectbiophysicsen_US
dc.subjectnanoscience & nanotechnologyen_US
dc.subjectphysics, fluids & plasmasen_US
dc.titleAntibody-Independent Isolation of Circulating Tumor Cells by Continuous-Flow Dielectrophoresisen_US
dc.typeArticleen_US

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