Genetic analysis of tendon development and musculoskeletal integration in the vertebrate head

dc.contributor.advisorEberhart, Johann K.
dc.contributor.committeeMemberWallingford, John
dc.contributor.committeeMemberVokes, Steven
dc.contributor.committeeMemberGordon, Vernita
dc.contributor.committeeMemberIyer, Vishwanath
dc.creatorMcGurk, Patrick Dooling
dc.creator.orcid0000-0002-6632-7423
dc.date.accessioned2017-12-12T16:02:36Z
dc.date.available2017-12-12T16:02:36Z
dc.date.created2017-08
dc.date.issued2017-08-11
dc.date.submittedAugust 2017
dc.date.updated2017-12-12T16:02:36Z
dc.description.abstractWe investigated zebrafish mutants that affect craniofacial development to examine how those mutations affect the musculoskeletal patterning of the jaw. We discovered that loss of function in cyp26b1 caused musculoskeletal patterning defects related to tendon condensation. Subsequently, we decided to use reverse genetics to interrogate regulation of tendon development in the vertebrate head and body. Needing ways of evaluating tendon and myotendinous junction phenotypes, we devised a protocol for inducing muscle strain in vivo. We generated mutants for scxa and xirp2a, determined whether homozygous mutants had developmental defects, and tested their muscle attachment stability under strain. In conjunction with our mutagenesis efforts, I developed a software program that would aid in the identification of mutant alleles in the F1 offspring of CRISPR mutagenized animals.
dc.description.departmentCellular and Molecular Biology
dc.format.mimetypeapplication/pdf
dc.identifierdoi:10.15781/T2FF3MG6B
dc.identifier.urihttp://hdl.handle.net/2152/62969
dc.language.isoen
dc.subjectZebrafish
dc.subjectDevelopment
dc.subjectGenetics
dc.subjectCRiSPR
dc.subjectMuscle
dc.subjectTendon
dc.subjectRetinoic acid
dc.subjectCyp26b1
dc.subjectLive imaging
dc.titleGenetic analysis of tendon development and musculoskeletal integration in the vertebrate head
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentCellular and Molecular Biology
thesis.degree.disciplineCell and Molecular Biology
thesis.degree.grantorThe University of Texas at Austin
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy

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