Investigation of yeast mitochondrial transcription initiation factor Mtf1p

dc.contributor.advisorYin, Yuhui Whitney
dc.creatorKlein, Dawn Elizabeth
dc.date.accessioned2017-04-10T18:32:48Z
dc.date.available2017-04-10T18:32:48Z
dc.date.issued2007-08
dc.description.abstractBeing the first step in gene expression, transcription is a tightly regulated process in the cell cycle. In humans, mitochondrial transcription defects have been associated with numerous diseases. In order to gain a greater understanding of mitochondrial transcription initiation in higher eukaryotes, the mitochondrial transcription initiation factor, Mtf1p, from Saccharomyces cerevisiae was studied. This protein, which has homologues in all other eukaryotes, is known to interact with Rpo41p, the core yeast mitochondrial RNA polymerase, prior to promoter-DNA binding. Four mutants were created, Mtf1p Y54F, C192A, C192F, and C192M, and then expressed in Escherichia coli cells. Initially, two mutants (C192F and C192M) were successfully expressed with an N-terminal GST-tag, and Mtf1p C192F was used to optimize an overall purification scheme. Gel filtration analysis revealed that after purification, Mtf1p C192F is in an aggregated state, and as a result, Mtf1p C192F was N-terminally His-tagged in an alternative vector. However, expression was of the His-tagged protein was again unsuccessful. At the time this thesis was written, experiments to express the four Mtf1p mutants are ongoing.en_US
dc.description.departmentBiochemistryen_US
dc.format.mediumelectronicen_US
dc.identifierdoi:10.15781/T22Z12V55
dc.identifier.urihttp://hdl.handle.net/2152/46382
dc.language.isoengen_US
dc.relation.ispartofUT Electronic Theses and Dissertationsen_US
dc.rightsCopyright © is held by the author. Presentation of this material on the Libraries' web site by University Libraries, The University of Texas at Austin was made possible under a limited license grant from the author who has retained all copyrights in the works.en_US
dc.rights.restrictionRestricteden_US
dc.subjectMitochondrial transcription initiationen_US
dc.subjectHigher eukaryotesen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.titleInvestigation of yeast mitochondrial transcription initiation factor Mtf1pen_US
dc.typeThesisen_US
dc.type.genreThesisen_US
thesis.degree.departmentBiochemistryen_US
thesis.degree.disciplineBiochemistryen_US
thesis.degree.grantorUniversity of Texas at Austinen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Artsen_US
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