Strain engineering for improved expression of recombinant proteins in bacteria

dc.creatorMakino, Tomohiroen
dc.creatorSkretas, Georgiosen
dc.creatorGeorgiou, Georgeen
dc.descriptionTomohiro Makino and George Georgiou are with the Department of Chemical Engineering, The University of Texas at Austin and the Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712, USA -- George Gerorgiou is with the Department of Biomedical Engineering, The University of Texas at Austin and the Section of Molecular Genetics and Microbiology, The University of Texas at Austin, Austin, Texas 78712, USA -- Georgios Skretas is with the Insitute of Biological Research and Biotechnology, National Hellenic Research Foundation, 48 Vassileos Constantinou Ave., 11635 Athens, Greece -- Tomohiro Makino is with the Asubio Pharma CO., LTD. 6-4-3, Minatojima-Minamimachi Chuo-ku, Kobe 650-0047, Japanen
dc.description.abstractProtein expression in Escherichia coli represents the most facile approach for the preparation of non-glycosylated proteins for analytical and preparative purposes. So far, the optimization of recombinant expression has largely remained a matter of trial and error and has relied upon varying parameters, such as expression vector, media composition, growth temperature and chaperone co-expression. Recently several new approaches for the genome-scale engineering of E. coli to enhance recombinant protein expression have been developed. These methodologies now enable the generation of optimized E. coli expression strains in a manner analogous to metabolic engineering for the synthesis of low-molecular-weight compounds. In this review, we provide an overview of strain engineering approaches useful for enhancing the expression of hard-to-produce proteins, including heterologous membrane proteins.en
dc.description.departmentChemical Engineeringen
dc.description.departmentInstitute for Cellular and Molecular Biologyen
dc.description.departmentBiomedical Engineeringen
dc.description.departmentMolecular Biosciencesen
dc.identifier.citationMakino, Tomohiro, Georgios Skretas, and George Georgiou. “Strain Engineering for Improved Expression of Recombinant Proteins in Bacteria.” Microbial Cell Factories 10, no. 1 (May 14, 2011): 32. doi:10.1186/1475-2859-10-32.en
dc.publisherMicrobial Cell Factoriesen
dc.rightsAdministrative deposit of works to UT Digital Repository: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access at The public license is specified as CC-BY: The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en
dc.subjectEscherichia colien
dc.subjectnon-glycosylated proteinsen
dc.subjectgenome-scale engineeringen
dc.subjectheterologous membrane proteinsen
dc.titleStrain engineering for improved expression of recombinant proteins in bacteriaen

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