Cytoplasmic foci at the crossroads of artifactual science and biological function

dc.contributor.advisorMarcotte, Edward M.en
dc.contributor.committeeMemberEllington, Andrew Den
dc.contributor.committeeMemberZhang, Yan Jen
dc.contributor.committeeMemberAppling, Dean Ren
dc.contributor.committeeMemberIyer, Vishwanath Ren
dc.creatorZhao, Aliceen
dc.date.accessioned2016-08-23T21:15:39Z
dc.date.available2016-08-23T21:15:39Z
dc.date.issued2016-05
dc.date.submittedMay 2016
dc.date.updated2016-08-23T21:15:39Z
dc.description.abstractDeciphering protein interaction and compartmentalization is crucial to understanding the molecular mechanisms that drive biological processes. Using various high throughput approaches, we have managed to score subcellular dynamic protein re-organization into supramolecular structures and map physical association networks to discover protein complexes on a proteome-wide level. However, the case by case studies of some of these novel structures and interactions reveal difficulties in interpreting their biological basis. This study offers insights into limits inherent in the molecular techniques used to investigate subcellular structures and protein interactions, describing a set of cautionary tales and critical analysis for deciphering cases of confounding data from orthogonal approaches. This study also offers a new experimental technique for high-throughput imaging assays with mammalian cell lines.en
dc.description.departmentCellular and Molecular Biologyen
dc.format.mimetypeapplication/pdfen
dc.identifierdoi:10.15781/T2QR4NQ99en
dc.identifier.urihttp://hdl.handle.net/2152/39610en
dc.language.isoenen
dc.subjectProteinen
dc.subjectFocien
dc.subjectFiberen
dc.subjectFilamenten
dc.subjectMacrostructureen
dc.subjectImmunofluorescenceen
dc.subjectImmunoprecipitationen
dc.subjectMicroscopyen
dc.subjectProtein tagen
dc.subjectTTC4en
dc.subjectPurinosomeen
dc.subjectCo-fractionationen
dc.subjectCell chipen
dc.subjectTransfectionen
dc.subjectHigh-throughputen
dc.subjectAggregationen
dc.subjectCTP synthaseen
dc.subjectGlutamine synthetaseen
dc.subjectPurine biosynthesisen
dc.titleCytoplasmic foci at the crossroads of artifactual science and biological functionen
dc.typeThesisen
dc.type.materialtexten
thesis.degree.departmentCellular and Molecular Biologyen
thesis.degree.disciplineCell and molecular biologyen
thesis.degree.grantorThe University of Texas at Austinen
thesis.degree.levelDoctoralen
thesis.degree.nameDoctor of Philosophyen

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