Rapid isolation of monoclonal antibodies from animals

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Date

2015-07-28

Authors

George M. Georgiou
Andrew D. Ellington
Edward M. Marcotte
Sai T. Reddy
Xin Ge
Jason Lavinder
Daniel Boutz

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United States Patent and Trademark Office

Abstract

Methods and compositions for identification of candidate antigen-specific variable regions as well as generation of antibodies or antigen-binding fragments that could have desired antigen specificity are provided. For example, in certain aspects methods for determining amino acid sequences of serum antibody CDR and abundancy level are described. In some aspects, methods for determining nucleic acid sequences of antibody variable region sequences and frequency are provided. Furthermore, the invention provides methods for identification and generation of antibody or antigen-binding fragments that comprise highly-represented CDR.

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