In vitro polyketide biocatalysis : triketide building-blocks and enzymology

dc.contributor.advisorKeatinge-Clay, Adrian Tristan
dc.creatorHarper, Andrew Daviden
dc.date.accessioned2013-10-08T15:30:09Zen
dc.date.issued2013-05en
dc.date.submittedMay 2013en
dc.date.updated2013-10-08T15:30:10Zen
dc.descriptiontexten
dc.description.abstractPolyketide products are useful compounds to research and industry but can be difficult to access due to their richness in stereogenic centers. Type I polyketide synthases offer unique engineering opportunities to access natural stereocontrol and resultant complex compounds. The development of a controlled in vitro platform based around type I polyketide synthases is described. It has been used to produce a small library of polyketide fragments on an unprecedented and synthetically-relevant scale and explore polyketide synthase enzymology.en
dc.description.departmentCellular and Molecular Biologyen
dc.format.mimetypeapplication/pdfen
dc.identifier.urihttp://hdl.handle.net/2152/21485en
dc.language.isoen_USen
dc.subjectIn vitroen
dc.subjectCell-freeen
dc.subjectPreparativeen
dc.subjectPolyketideen
dc.subjectPolyketide synthaseen
dc.subjectKetoreductaseen
dc.subjectMalonyl-CoA ligaseen
dc.subjectMatBen
dc.subjectEnzymologyen
dc.subjectChiral building blocksen
dc.subjectChiral synthonsen
dc.subjectDiketideen
dc.subjectTriketideen
dc.subjectLactoneen
dc.subjectKetolactoneen
dc.subjectNatural productsen
dc.subjectSecondary metabolismen
dc.subjectSecondary metaboliteen
dc.subjectModularen
dc.subjectType I polyketide synthaseen
dc.subjectBiosynthesisen
dc.subjectStreptomyces coelicoloren
dc.subjectSaccharopolyspora erythraeaen
dc.subjectBacillus subtilisen
dc.subjectStreptomyces nodosusen
dc.subjectSaccharomyces cerevisiaeen
dc.subjectStreptomyces antibioticusen
dc.subjectStreptomyces venezuelaeen
dc.subjectSaccharopolyspora spinosaen
dc.subjectStreptomyces fradiaeen
dc.titleIn vitro polyketide biocatalysis : triketide building-blocks and enzymologyen
thesis.degree.departmentCellular and Molecular Biologyen
thesis.degree.disciplineCell and Molecular Biologyen
thesis.degree.grantorThe University of Texas at Austinen
thesis.degree.levelMastersen
thesis.degree.nameMaster of Artsen

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