In vitro polyketide biocatalysis : triketide building-blocks and enzymology
Date
2013-05
Authors
Harper, Andrew David
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Abstract
Polyketide products are useful compounds to research and industry but can be difficult to access due to their richness in stereogenic centers. Type I polyketide synthases offer unique engineering opportunities to access natural stereocontrol and resultant complex compounds. The development of a controlled in vitro platform based around type I polyketide synthases is described. It has been used to produce a small library of polyketide fragments on an unprecedented and synthetically-relevant scale and explore polyketide synthase enzymology.
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Keywords
In vitro , Cell-free , Preparative , Polyketide , Polyketide synthase , Ketoreductase , Malonyl-CoA ligase , MatB , Enzymology , Chiral building blocks , Chiral synthons , Diketide , Triketide , Lactone , Ketolactone , Natural products , Secondary metabolism , Secondary metabolite , Modular , Type I polyketide synthase , Biosynthesis , Streptomyces coelicolor , Saccharopolyspora erythraea , Bacillus subtilis , Streptomyces nodosus , Saccharomyces cerevisiae , Streptomyces antibioticus , Streptomyces venezuelae , Saccharopolyspora spinosa , Streptomyces fradiae