Expression and Purification of Calmodulin and its Interaction with the SK peptide

dc.contributor.advisorAldrich, Richard
dc.creatorAbraham, Jonathan
dc.date.accessioned2018-05-14T19:20:33Z
dc.date.available2018-05-14T19:20:33Z
dc.date.issued2018-05-04
dc.description.abstractCalmodulin (CaM) is a highly conserved protein that has been observed to regulate ion channels. Calcium (Ca2+) binds to Calmodulin in order to activate Ca2+ activated Potassium channels (SK Channels). Calmodulin is known to responsible for gating the SK channel; however, the mechanism in which it does this is unclear. In order to study this interaction, wild-type CaM, single lobe or half CaM, double lobe CaM, and a SK channel peptide (SKp) were all purified. Purification of proteins was done through high performance liquid chromatography (HPLC) and verified with SDS gels. The CaM-SKp complex was analyzed through CG-MALS (composition gradient multi-angle light scattering). CG-MALS can be used to determine the molar mass of complexes formed. Stoichiometry can be determined if molar mass of the proteins complexing are known. Results from CG-MALS show the formation of stoichiometries that have not been observed before. The introduction of these new stoichiometries adds complexity to the SKp-CaM interaction and require more testing in order to fully elucidate the mechanism of SK channel gating.en_US
dc.description.departmentBiochemistryen_US
dc.identifierdoi:10.15781/T2KD1R347
dc.identifier.urihttp://hdl.handle.net/2152/65164
dc.language.isoengen_US
dc.relation.ispartofHonors Thesesen_US
dc.rights.restrictionOpenen_US
dc.subjectcalmodulinen_US
dc.subjectSK channelen_US
dc.subjectcalcium signalingen_US
dc.subjectlight scatteringen_US
dc.subjecthigh performance liquid chromatography (HPLC)en_US
dc.subjectHPLCen_US
dc.titleExpression and Purification of Calmodulin and its Interaction with the SK peptideen_US
dc.typeThesisen_US

Access full-text files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Abraham_Jonathan.pdf
Size:
1.85 MB
Format:
Adobe Portable Document Format
Description:

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.66 KB
Format:
Item-specific license agreed upon to submission
Description:

Collections