Cross-Seeding Controls A beta Fibril Populations and Resulting Functions
| dc.creator | Lucas, Michael J. | |
| dc.creator | Pan, Henry S. | |
| dc.creator | Verbeke, Eric J. | |
| dc.creator | Partipilo, Gina M. | |
| dc.creator | Helfman, Ethan C. | |
| dc.creator | Kann, Leah | |
| dc.creator | Keitz, Benjamin K. | |
| dc.creator | Taylor, David W. | |
| dc.creator | Webb, Lauren J. | |
| dc.date.accessioned | 2024-02-08T16:12:57Z | |
| dc.date.available | 2024-02-08T16:12:57Z | |
| dc.date.issued | 2021-10-14 | |
| dc.description.abstract | Amyloid peptides nucleate from monomers to aggregate into fibrils through primary nucleation. Pre-existing fibrils can then act as seeds for additional monomers to fibrillize through secondary nucleation. Both nucleation processes occur simultaneously, yielding a distribution of fibril polymorphs that can generate a spectrum of neurodegenerative effects. Understanding the mechanisms driving polymorph structural distribution during both nucleation processes is important for uncovering fibril structure-function relationships, as well as for creating polymorph distributions in vitro that better match fibril structures found in vivo. Here, we explore how cross-seeding wild-type (WT) Aβ1-40 with Aβ1-40 mutants E22G (Arctic) and E22Δ (Osaka), as well as with WT Aβ1-42, affects the distribution of fibril structural polymorphs and how changes in structural distribution impact toxicity. Transmission electron microscopy analysis revealed that fibril seeds derived from mutants of Aβ1-40 imparted their structure to WT Aβ1-40 monomers during secondary nucleation, but WT Aβ1-40 fibril seeds do not affect the structure of fibrils assembled from mutant Aβ1-40 monomers, despite the kinetic data indicating accelerated aggregation when cross-seeding of any combination of mutants. Additionally, WT Aβ1-40 fibrils seeded with mutant fibrils produced similar structural distributions to the mutant seeds with similar cytotoxicity profiles. This indicates that mutant fibril seeds not only impart their structure to growing WT Aβ1-40 aggregates but also impart cytotoxic properties. Our findings establish a relationship between the fibril structure and the phenotype on a polymorph population level and that these properties can be passed on through secondary nucleation to the succeeding generations of fibrils. | |
| dc.description.department | Center for Dynamics and Control of Materials | |
| dc.description.sponsorship | This work was supported in part by Welch Foundation Research Grants F-1722 (to L.J.W.) and F-1929 (to B.K.K.); National Science Foundation CHE-1807215 (to L.J.W.); and the College of Natural Sciences at The University of Texas at Austin (Catalyst Grant to L.J.W., B.K.K., and D.W.T.). Partial support was provided by the National Science Foundation through the Center for Dynamics and Control of Materials: an NSF Materials Research Science and Engineering Center under DMR-1720595. D.W.T. is a CPRIT Scholar supported by the Cancer Prevention and Research Institute of Texas (RR160088) and an Army Young Investigator supported by the Army Research Office (W911NF-19-1-0021). | |
| dc.identifier.doi | https://doi.org/10.1101/2021.10.14.464427 | |
| dc.identifier.uri | https://hdl.handle.net/2152/123629 | |
| dc.identifier.uri | https://doi.org/10.26153/tsw/50423 | |
| dc.language.iso | en_US | |
| dc.relation.ispartof | Center for Dynamics and Control of Materials Publications | |
| dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | en |
| dc.rights.restriction | Open | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | |
| dc.subject | Fibril | |
| dc.title | Cross-Seeding Controls A beta Fibril Populations and Resulting Functions | |
| dc.type | Article |