Discovery of blood-brain barrier penetrating peptides by phage display technology to deliver biologics into the brain

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dc.contributor.advisorGhosh, Debadyuti
dc.contributor.committeeMemberWilliams, Robert O.
dc.contributor.committeeMemberCui, Zhengrong
dc.contributor.committeeMemberBaker, Aaron B.
dc.creatorPeng, Xiujuan
dc.date.accessioned2021-10-01T22:23:59Z
dc.date.available2021-10-01T22:23:59Z
dc.date.created2019-08
dc.date.issued2019-09-19
dc.date.submittedAugust 2019
dc.date.updated2021-10-01T22:24:00Z
dc.description.abstractBiologics as macromolecules can’t deliver to the brain without the blood-brain barrier (BBB) transport carriers. A phage display peptide library was employed to screen the BBB shuttling peptides by biopanning against an in vitro BBB model. We identified several novel and efficient BBB permeable peptides. A series of in vivo and in vitro studies were conducted to validate the capability of these peptides to transport the BBB and the brain extracellular matrix (ECM). The findings demonstrated that our peptides, Pep-3 and Pep-9 are able to shuttle across BBB and deliver to the brain parenchyma in vivo. Thereafter, Pep-9 was selected to develop IgG-Pep-9 conjugate formulation by click chemistry. In vivo delivery of IgG-Pep-9 indicated that Pep-9 can carry antibody molecules to transport BBB and deliver to the brain parenchyma.
dc.description.departmentPharmaceutical Sciences
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/2152/88323
dc.identifier.urihttp://dx.doi.org/10.26153/tsw/15262
dc.language.isoen
dc.subjectBlood brain barrier
dc.subjectPhage display
dc.subjectPeptide
dc.subjectAntibody
dc.titleDiscovery of blood-brain barrier penetrating peptides by phage display technology to deliver biologics into the brain
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentPharmaceutical Sciences
thesis.degree.disciplinePharmaceutical Sciences
thesis.degree.grantorThe University of Texas at Austin
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy

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