A Low Concentration of Ethanol Impairs Learning but Not Motor and Sensory Behavior in Drosophila Larvae

dc.creatorRobinson, Brooks G.en
dc.creatorKhurana, Sukanten
dc.creatorPohl, Jascha B.en
dc.creatorLi, Wen-keen
dc.creatorGhezzi, Alfredoen
dc.creatorCady, Amanda M.en
dc.creatorNajjar, Kristinaen
dc.creatorHatch, Michael M.en
dc.creatorShah, Ruchita R.en
dc.creatorBhat, Amaren
dc.creatorHariri, Omaren
dc.creatorHaroun, Kareem B.en
dc.creatorYoung, Melvin C.en
dc.creatorFife, Kathrynen
dc.creatorHooten, Jeffen
dc.creatorTran, Tuanen
dc.creatorGoan, Danielen
dc.creatorDesai, Foramen
dc.creatorHusain, Farhanen
dc.creatorGodinez, Ryan M.en
dc.creatorSun, Jeffrey C.en
dc.creatorCorpuz, Jonathanen
dc.creatorMoran, Jacxelynen
dc.creatorZhong, Allen C.en
dc.creatorChen, William Y.en
dc.creatorAtkinson, Nigel S.en
dc.date.accessioned2013-05-23T16:22:13Zen
dc.date.available2013-05-23T16:22:13Zen
dc.date.issued2012-05-18en
dc.description.abstractDrosophila melanogaster has proven to be a useful model system for the genetic analysis of ethanol-associated behaviors. However, past studies have focused on the response of the adult fly to large, and often sedating, doses of ethanol. The pharmacological effects of low and moderate quantities of ethanol have remained understudied. In this study, we tested the acute effects of low doses of ethanol (~7 mM internal concentration) on Drosophila larvae. While ethanol did not affect locomotion or the response to an odorant, we observed that ethanol impaired associative olfactory learning when the heat shock unconditioned stimulus (US) intensity was low but not when the heat shock US intensity was high. We determined that the reduction in learning at low US intensity was not a result of ethanol anesthesia since ethanol-treated larvae responded to the heat shock in the same manner as untreated animals. Instead, low doses of ethanol likely impair the neuronal plasticity that underlies olfactory associative learning. This impairment in learning was reversible indicating that exposure to low doses of ethanol does not leave any long lasting behavioral or physiological effects.en
dc.description.departmentNeuroscienceen
dc.description.sponsorshipThis work was funded by NIH R01 AA018037 to NSA and NSF grant IOS-0641370 to NSA supported the undergraduate contribution. BGR and JBP were supported by the NIH Ruth L. Kirschstein National Research Service Award Institutional Research Training Grant # 5T32AA007471. AC and RG received The University of Texas Undergraduate Research Fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.identifier.citationRobinson BG, Khurana S, Pohl JB, Li W-k, Ghezzi A, et al. (2012) A Low Concentration of Ethanol Impairs Learning but Not Motor and Sensory Behavior in Drosophila Larvae. PLoS ONE 7(5): e37394. doi:10.1371/journal.pone.0037394en
dc.identifier.doi10.1371/journal.pone.0037394en
dc.identifier.urihttp://hdl.handle.net/2152/20154en
dc.language.isoengen
dc.publisherPublic Library of Scienceen
dc.rightsAttribution 3.0 United Statesen
dc.rightsCC-BYen
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/en
dc.subjectAnimal behavioren
dc.subjectConditioned responseen
dc.subjectDrosophilaen
dc.subjectDrosophila melanogasteren
dc.subjectEthanolen
dc.subjectHeat shocken
dc.subjectLarvaeen
dc.subjectLearningen
dc.titleA Low Concentration of Ethanol Impairs Learning but Not Motor and Sensory Behavior in Drosophila Larvaeen
dc.typeArticleen

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