Solid-Like Domains in Mixed Lipid Bilayers: Effect of Membrane Lamellarity and Transition Pathway

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Date

2014

Authors

Gordon, Vernita D.
Beales, Paul A.
Shearman, Gemma C.
Zhao, Zhijun
Seddon, John M.
Poon, Wilson C. K.
Egelhaaf, Stefan U.

Journal Title

Journal ISSN

Volume Title

Publisher

Advances in Planar Lipid Bilayers and Liposomes

Abstract

We present optical observations of phase separation in mixed model membranes in the form of giant unilamellar vesicles. These observations are compared to the phase behavior of lipid mixtures, which we determined by X-ray scattering and differential scanning calorimetry or extracted from the existing literature. The domain properties are affected not only by the bulk phase behavior but also by the membrane lamellarity and phase transition pathways. These observations have important implications for how phase behavior determined by bulk methods using dense, multilamellar lipid bilayers are linked to phase separation in giant, unilamellar lipid bilayers as observed by microscopy.

Description

*SUPA, School of Physics, and Collaborative Optical Spectroscopy, Micromanipulation and Imaging Centre (COSMIC), The University of Edinburgh, Edinburgh, Scotland, United Kingdom †Department of Chemistry, Imperial College London, South Kensington, London, United Kingdom {Condensed Matter Physics Laboratory, Heinrich-Heine-University, Du¨ sseldorf, Germany 1Present Address: Department of Physics and Center for Nonlinear Dynamics, University of Texas at Austin, Austin, USA 2Present Address: Centre for Molecular Nanoscience, School of Chemistry, University of Leeds, Leeds LS2 9JT, United Kingdom 3Present Address: Molecular and Pharmaceutical Science, London Metropolitan University, London N7 8DB, United Kingdom 4Corresponding author: e-mail address: gordon@chaos.utexas.edu

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Citation

Gordon, Vernita D., Paul A. Beales, Gemma C. Shearman, Zhijun Zhao, John M. Seddon, Wilson CK Poon, and S. U. Egelhaaf. "Solid-Like Domains in Mixed Lipid Bilayers: Effect of Membrane Lamellarity and Transition Pathway." Adv. Planar Lipid Bilayers Liposomes (2014): 137-153.