Well Plate Circular Dichroism Reader for the Rapid Determination of Enantiomeric Excess

dc.contributor.utaustinauthorMetola, P.en_US
dc.contributor.utaustinauthorAnslyn, E. V.en_US
dc.creatorMetola, P.en_US
dc.creatorNichols, S. M.en_US
dc.creatorKahr, B.en_US
dc.creatorAnslyn, E. V.en_US
dc.date.accessioned2016-09-23T18:07:46Z
dc.date.available2016-09-23T18:07:46Z
dc.date.issued2014-07en_US
dc.description.abstractCircular dichroism (CD) spectropolarimeters typically employ one photoelastic modulator. However, spectropolarimeters employing two or even four modulators are more versatile and can be used to subvert common measurement errors arising from imperfectly isotropic samples or sample holders. Small linear anisotropies that can cause large errors in CD measurement can be associated with multi-well sample holders. Thus, high-throughput CD analyses in multi-well plates have not yet been demonstrated. One such application is the determination of enantiomeric excess of a library of reaction products. Herein, a spectropolarimeter employing four photoelastic modulators and a translation stage was used to determine the enantiomeric excess of a family of chiral amine complexes much more rapidly than could be achieved with a robotic fluid injection system. These experiments are proof of concept for high-throughput CD analysis. In practice, commercially available glass bottomed well plates are sufficiently strain free that a simple instrument with just one photoelastic modulator and a vertical optical train should be able to deliver the CD without special considerations given herein. On the other hand, polystyrene well plates cannot be used in this way.en_US
dc.description.departmentChemistryen_US
dc.description.sponsorshipNational Institutes of Health GM077437en_US
dc.description.sponsorshipWelch Foundation F-1151en_US
dc.description.sponsorshipNational Science Foundation DMR-1105000, DGE-12342536en_US
dc.identifierdoi:10.15781/T2PK0733T
dc.identifier.citationMetola, P., S. M. Nichols, Bart Kahr, and E. V. Anslyn. "Well plate circular dichroism reader for the rapid determination of enantiomeric excess." Chemical Science 5, no. 11 (Jul., 2014): 4278-4282.en_US
dc.identifier.doi10.1039/c4sc01641fen_US
dc.identifier.issn2041-6520en_US
dc.identifier.urihttp://hdl.handle.net/2152/41182
dc.language.isoEnglishen_US
dc.relation.ispartofen_US
dc.relation.ispartofserialChemical Scienceen_US
dc.rightsAdministrative deposit of works to Texas ScholarWorks: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access or the publisher allows a PDF version of the article to be freely posted online. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en_US
dc.rights.restrictionOpenen_US
dc.subjectthroughput screening assaysen_US
dc.subjectasymmetric hydrogenationen_US
dc.subjectparallelen_US
dc.subjectsynthesisen_US
dc.subjectenantioselective catalysisen_US
dc.subjectligand librariesen_US
dc.subjectchiral ligandsen_US
dc.subjectphosphoramiditeen_US
dc.subjectcombinatorialen_US
dc.subjectspectroscopyen_US
dc.subjectdiscoveryen_US
dc.subjectchemistry, multidisciplinaryen_US
dc.titleWell Plate Circular Dichroism Reader for the Rapid Determination of Enantiomeric Excessen_US
dc.typeArticleen_US

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