The behavior of proteins at solid-liquid interfaces
| dc.contributor.advisor | Anslyn, Eric V., 1960- | |
| dc.creator | Garland, Adam Till | en |
| dc.date.accessioned | 2014-07-07T16:17:28Z | en |
| dc.date.issued | 2014-05 | en |
| dc.date.submitted | May 2014 | en |
| dc.date.updated | 2014-07-07T16:17:28Z | en |
| dc.description | text | en |
| dc.description.abstract | The behavior of a protein molecule at the solid-liquid interface is a worthy scientific problem for at least three reasons. The main driving force for studying this problem is a practical one, as many areas of bio-related technologies, such as medical implants, biosensing, and drug delivery, require the understanding of protein-surface interactions. In this dissertation, the nature of the precursive weakly adsorbed state of proteins during binding is reviewed. From this perspective, the adsorption and binding of proteins to a solid block copolymer thin film was achieved with regular spacing. Further efforts produced a monolayer of green fluorescent protein (GFP) covalently bound with regular spacing and orientation to a diblock copolymer thin film. This protein could be folded and refolded by changing solvent characteristics. We also explored the binding of DC-SIGN to mannose and mannotriose bearing lipid membranes. While no binding was observed, the usefulness of the lipid-based glycan microarray was proven using the well-studied CTB-GM1 binding motif. | en |
| dc.description.department | Chemistry | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.uri | http://hdl.handle.net/2152/25034 | en |
| dc.language.iso | en | en |
| dc.subject | Diblock copolymer | en |
| dc.subject | Protein adsorption | en |
| dc.subject | Lipid bilayer | en |
| dc.subject | DC-SIGN | en |
| dc.title | The behavior of proteins at solid-liquid interfaces | en |
| dc.type | Thesis | en |
| thesis.degree.department | Chemistry | en |
| thesis.degree.discipline | Chemistry | en |
| thesis.degree.grantor | The University of Texas at Austin | en |
| thesis.degree.level | Doctoral | en |
| thesis.degree.name | Doctor of Philosophy | en |