Rheological, In Situ Printability and Cell Viability Analysis of Hydrogels for Muscle Tissue Regeneration

dc.creatorRamesh, Srikanthan
dc.creatorGerdes, Sam
dc.creatorLau, Sharon
dc.creatorMostafavi, Azadeh
dc.creatorKong, Zhenyu
dc.creatorJohnson, Blake N.
dc.creatorTamayol, Ali
dc.creatorRao, Prahalada
dc.creatorRivero, Iris V.
dc.date.accessioned2021-11-09T20:35:48Z
dc.date.available2021-11-09T20:35:48Z
dc.date.issued2018
dc.description.abstractAdvancements in additive manufacturing have made it possible to fabricate biologically relevant architectures from a wide variety of materials. Hydrogels have garnered increased attention for the fabrication of muscle tissue engineering constructs due to their resemblance to living tissue and ability to function as cell carriers. However, there is a lack of systematic approaches to screen bioinks based on their inherent properties, such as rheology, printability and cell viability. Furthermore, this study takes the critical first-step for connecting in-process sensor data with construct quality by studying the influence of printing parameters. Alginate-chitosan hydrogels were synthesized and subjected to a systematic rheological analysis. In situ print layer photography was utilized to identify the optimum printing parameters and also characterize the fabricated three-dimensional structures. Additionally, the scaffolds were seeded with C2C12 mouse myoblasts to test the suitability of the scaffolds for muscle tissue engineering. The results from the rheological analysis and print layer photography led to the development of a set of optimum processing conditions that produced a quality deposit while the cell viability tests indicated the suitability of the hydrogel for muscle tissue engineering applications.en_US
dc.description.departmentMechanical Engineeringen_US
dc.identifier.urihttps://hdl.handle.net/2152/90160
dc.identifier.urihttp://dx.doi.org/10.26153/tsw/17081
dc.language.isoengen_US
dc.publisherUniversity of Texas at Austinen_US
dc.relation.ispartof2018 International Solid Freeform Fabrication Symposiumen_US
dc.rights.restrictionOpenen_US
dc.subjectprintabilityen_US
dc.subjectmuscle regenerationen_US
dc.subjectin-situ monitoringen_US
dc.subjectbioprintingen_US
dc.subjectC2C12 myoblastsen_US
dc.titleRheological, In Situ Printability and Cell Viability Analysis of Hydrogels for Muscle Tissue Regenerationen_US
dc.typeConference paperen_US
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