Mesenchymal Stem Cell Response to TGF-Beta 1 in Both 2D and 3D Environments

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dc.contributor.utaustinauthorStowers, Ryan S.en_US
dc.contributor.utaustinauthorDrinnan, Charles T.en_US
dc.contributor.utaustinauthorChung, Eunna N.en_US
dc.contributor.utaustinauthorSuggs, Laura J.en_US
dc.creatorStowers, Ryan S.en_US
dc.creatorDrinnan, Charles T.en_US
dc.creatorChung, Eunna N.en_US
dc.creatorSuggs, Laura J.en_US
dc.date.accessioned2016-10-28T19:52:44Z
dc.date.available2016-10-28T19:52:44Z
dc.date.issued2013en_US
dc.description.abstractSmooth muscle cells (SMC) are critical in stabilizing developing vascular networks, and transforming growth factor beta 1 (TGF-beta 1) has been shown to promote SMC differentiation from stem cells. Previously, our lab has developed a chemically modified fibrin-based hydrogel that induces endothelial cell (EC) phenotype and network formation from human mesenchymal stem cells (hMSCs) without exogenous cytokines. Additionally, we have shown that this hydrogel system is capable of releasing growth factors in a controlled manner. In the present work, the effects of TGF-beta 1 on hMSCs in both monolayer and fibrin-based gel culture systems were demonstrated. The objective was to enhance SMC properties through TGF-beta 1 signaling for vessel stability while maintaining EC gene expression and morphology. Proliferation was decreased with higher TGF-beta 1 concentration in both monolayer and 3D gel cultures. EC genes were predominantly downregulated in the presence of TGF-beta 1 in monolayer cultures, while SMC genes were generally upregulated. In fibrin-based gels, several SMC genes were significantly upregulated at high concentrations of TGF-beta 1. Even at elevated TGF-beta 1 concentrations, no significant differences were seen in EC genes for hMSCs in gels compared to controls. Network formation and growth occurred in PEGylated fibrin gels loaded with TGF-beta 1 and were not significantly different from gels without loaded growth factor. Additionally, production of smooth muscle a-actin (SMA) was significantly increased in gels loaded with TGF-beta 1. These results demonstrate a simultaneous response of hMSCs to both the 3D biomatrix and cytokine signaling cues.en_US
dc.description.departmentBiomedical Engineeringen_US
dc.description.sponsorshipAmerican Heart Associationen_US
dc.description.sponsorshipNational Science Foundationen_US
dc.description.sponsorshipCBET-0853996 ARRAen_US
dc.identifierdoi:10.15781/T2ZP3W33B
dc.identifier.citationStowers, Ryan S., Charles T. Drinnan, Eunna Chung, and Laura J. Suggs. "Mesenchymal stem cell response to TGF-?1 in both 2D and 3D environments." Biomaterials Science, Vol. 1, No. 8 (2013): 860-869.en_US
dc.identifier.doi10.1039/c3bm60057ben_US
dc.identifier.issn2047-4830en_US
dc.identifier.urihttp://hdl.handle.net/2152/43308
dc.language.isoEnglishen_US
dc.relation.ispartofen_US
dc.relation.ispartofserialBiomaterials Scienceen_US
dc.rightsAdministrative deposit of works to Texas ScholarWorks: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access or the publisher allows a PDF version of the article to be freely posted online. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en_US
dc.rights.restrictionOpenen_US
dc.subjectendothelial-like cellsen_US
dc.subjectsmooth-muscle-cellsen_US
dc.subjectin-vitroen_US
dc.subjecttransformingen_US
dc.subjectgrowth-factor-beta-1en_US
dc.subjectgrowth-factoren_US
dc.subjectprogenitor cellsen_US
dc.subjectgene-expressionen_US
dc.subjecttgf-betaen_US
dc.subjectdifferentiationen_US
dc.subjecttissueen_US
dc.subjectmaterials science, biomaterialsen_US
dc.titleMesenchymal Stem Cell Response to TGF-Beta 1 in Both 2D and 3D Environmentsen_US
dc.typeArticleen_US

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